Fabrication of Nonbiofouling Surface and Its Application to Surface Plasmon Field-Enhanced Fluorescence Spectroscopy
DC Field | Value | Language |
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dc.contributor.author | 김은경 | - |
dc.contributor.author | 이봉수 | - |
dc.contributor.author | 표현봉 | - |
dc.contributor.author | 송현우 | - |
dc.contributor.author | 김영필 | - |
dc.contributor.author | 최인성 | - |
dc.contributor.author | 김학성 | - |
dc.date.accessioned | 2022-10-07T10:16:39Z | - |
dc.date.available | 2022-10-07T10:16:39Z | - |
dc.date.created | 2022-09-19 | - |
dc.date.issued | 2008-06 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/171994 | - |
dc.description.abstract | Reducing the non-specific binding of proteins on a chip surface is essential for the development of biosensors with high sensitivity and specificity. We fabricated poly(oligo(ethylene glycol) methacrylate) (pOEGMA) films with various thicknesses as a nonbiofouling surface for surface plasmon field-enhanced fluorescence spectroscopy (SPFS). To accomplish a sensitive SPF signal, the thickness of the pOEGMA films was controlled by surface-initiated atom transfer radical polymerization (SI-ATRP) on gold-coated glass (Au/Glass) and gold-coated Si (Au/Si) substrates. When fluorophores immobilized on the pOEGMA films were directly excited without a plasmon effect, the fluorescence intensity displayed a linear dependency on the tested thickness of the pOEGMA films on both Au/Si (9-60 nm) and Au/Glass (8-56 nm). As a result, the surface plasmon fieldinduced fluorescence intensity on Au/Glass enabled thickness-dependent sensitivity. The SPF signal on 50-nm-thick pOEGMA films was estimated to be 100 -fold stronger than that on 13-nm-thick pOEGMA films. Since the SPFS on pOEGMA film can allow an easy conjugation of biomolecules and provide a high resistance to biofouling events, our fabricated system has a great potential as a platform for biosensors with high sensitivity. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | 한국바이오칩학회 | - |
dc.title | Fabrication of Nonbiofouling Surface and Its Application to Surface Plasmon Field-Enhanced Fluorescence Spectroscopy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 김영필 | - |
dc.identifier.scopusid | 2-s2.0-70449097455 | - |
dc.identifier.wosid | 000258694400003 | - |
dc.identifier.bibliographicCitation | BioChip Journal, v.2, no.2, pp.103 - 110 | - |
dc.relation.isPartOf | BioChip Journal | - |
dc.citation.title | BioChip Journal | - |
dc.citation.volume | 2 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 103 | - |
dc.citation.endPage | 110 | - |
dc.type.rims | ART | - |
dc.identifier.kciid | ART001364246 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology -Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | RESONANCE | - |
dc.subject.keywordPlus | FUNCTIONALIZATION | - |
dc.subject.keywordPlus | DISTANCE | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | GOLD | - |
dc.subject.keywordAuthor | Nonbiofouling surface | - |
dc.subject.keywordAuthor | Poly(oligo(ethylene glycol) methacrylate) films | - |
dc.subject.keywordAuthor | Surface plasmon field-enhanced fluorescence spectroscopy | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.identifier.url | https://www.biochips.or.kr/website/04web01.php?mode=vie&number=53&vol=2&num=2&keyfield=pressdate&key=20080620 | - |
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