Effect of sildenafil citrate on interleukin-1 beta-induced nitric oxide synthesis and iNOS expression in SW982 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kyung-Ok | - |
dc.contributor.author | Park, Shin-Young | - |
dc.contributor.author | Han, Chang-Woo | - |
dc.contributor.author | Chung, Hyun Kee | - |
dc.contributor.author | Ryu, Dae-Hyun | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.date.accessioned | 2022-10-07T10:28:11Z | - |
dc.date.available | 2022-10-07T10:28:11Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-06 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/172037 | - |
dc.description.abstract | The purpose of this study was to identify the effect of sildenafil citrate on IL-1 beta-induced nitric oxide (NO) synthesis and iNOS expression in human synovial sarcoma SW982 cells. IL-1 beta stimulated the cells to generate NO in both dose- and time-dependent manners. The IL-1 beta-induced NO synthesis was inhibited by guanylate cyclase (GC) inhibitor, LY83583. When the cells were treated with 8-bromo-cGMP, a hydrolyzable analog of cGMP, NO synthesis was increased upto 5-fold without IL-1 beta treatment suggesting that cGMP is an essential component for increasing the NO synthesis. Synoviocytes and chondrocytes contain strong cGMP phosphodiesterase (PDE) activity, which has biochemical features of PDE5. When SW982 cells were pretreated with sildenafil citrate (Viagra), a PDE5 specific inhibitor, sildenafil citrate significantly inhibited IL-1 beta-induced NO synthesis and iNOS expressions. From this result, we noticed that PDE5 activity is required for IL-1 beta-induced NO synthesis and iNOS expressions in human synovial sarcoma cells, and sildenafil citrate may be able to suppress an inflammatory reaction of synovium through inhibition of NO synthesis and iNOS expression by cytokines. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Effect of sildenafil citrate on interleukin-1 beta-induced nitric oxide synthesis and iNOS expression in SW982 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong-Soo | - |
dc.identifier.doi | 10.3858/emm.2008.40.3.286 | - |
dc.identifier.scopusid | 2-s2.0-46449092458 | - |
dc.identifier.wosid | 000257360800004 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.40, no.3, pp.286 - 293 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 40 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 286 | - |
dc.citation.endPage | 293 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001250604 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | RHEUMATOID-ARTHRITIS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CYCLIC-GMP | - |
dc.subject.keywordPlus | CGMP | - |
dc.subject.keywordPlus | PHOSPHODIESTERASE | - |
dc.subject.keywordPlus | PATHOPHYSIOLOGY | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | CAMP | - |
dc.subject.keywordAuthor | cyclic nucleotide phosphodiesterases, type 5 | - |
dc.subject.keywordAuthor | interleukin-1 beta | - |
dc.subject.keywordAuthor | nitric oxide | - |
dc.subject.keywordAuthor | nitric oxide synthase type II | - |
dc.subject.keywordAuthor | sildenafil | - |
dc.identifier.url | https://www.nature.com/articles/emm200833 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.