Trichomonas vaginalis: Reactive oxygen species mediates caspase-3 dependent apoptosis of human neutrophils
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Song, Hyun-Ouk | - |
dc.contributor.author | Shin, Myeong-Heon | - |
dc.contributor.author | Alin, Myoung-Hee | - |
dc.contributor.author | Min, Duk-Young | - |
dc.contributor.author | Kim, Yong-Seok | - |
dc.contributor.author | Ryu, Jae-Sook | - |
dc.date.accessioned | 2022-10-07T10:42:37Z | - |
dc.date.available | 2022-10-07T10:42:37Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-01 | - |
dc.identifier.issn | 0014-4894 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/172166 | - |
dc.description.abstract | There are many neutrophils in the vaginal discharge from women infected with Trichomonas vaginalis. The aim of our study was to determine whether human neutrophil apoptosis may be regulated by reactive oxygen species (ROS) in response to trichomonads infection. Incubation of human neutrophils with live trichomonads caused marked receptor shedding of CD16, decrease of mitochondrial membrane potential (MMP) and caspase-3 activation in human neutrophils. These proapoptotic effects of T. vaginalis on neutrophils were inhibited by pretreatment of neutrophils with an inhibitor of NADPH oxidase, diphenyleneiodonium chloride (I)PI), suggesting an important role of intracellular ROS accumulation in T. vaginalis-triggered apoptosis. Indeed, large amounts of ROS levels were detected in neutrophils incubated with live trichomonads, and were also effectively inhibited by DPI. However, pan-caspase inhibitor z-VAD-fmk or caspase-3 inhibitor z-DEVD-fmk did not affect T. vaginalis-induced ROS generation in neutrophils. These results suggest that ROS-dependent caspase-3 activation plays an important role in apoptosis of human neutrophils induced by T. vaginalis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Trichomonas vaginalis: Reactive oxygen species mediates caspase-3 dependent apoptosis of human neutrophils | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Yong-Seok | - |
dc.contributor.affiliatedAuthor | Ryu, Jae-Sook | - |
dc.identifier.doi | 10.1016/j.exppara.2007.06.010 | - |
dc.identifier.scopusid | 2-s2.0-37449018330 | - |
dc.identifier.wosid | 000252976200010 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL PARASITOLOGY, v.118, no.1, pp.59 - 65 | - |
dc.relation.isPartOf | EXPERIMENTAL PARASITOLOGY | - |
dc.citation.title | EXPERIMENTAL PARASITOLOGY | - |
dc.citation.volume | 118 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 59 | - |
dc.citation.endPage | 65 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | NADPH OXIDASE | - |
dc.subject.keywordPlus | CELL-DEATH | - |
dc.subject.keywordPlus | ACTIVATING CASPASE-3 | - |
dc.subject.keywordPlus | PROMOTES APOPTOSIS | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE | - |
dc.subject.keywordPlus | INTACT-CELLS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | INVOLVEMENT | - |
dc.subject.keywordAuthor | γ-Glu-Cys-Gly | - |
dc.subject.keywordAuthor | 2′7′-dichlorofluorescein-diacetate | - |
dc.subject.keywordAuthor | 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benz imidazolcarbocyanine iodide | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Caspase-3 | - |
dc.subject.keywordAuthor | DCF-DA | - |
dc.subject.keywordAuthor | diphenyleneiodonium chloride | - |
dc.subject.keywordAuthor | DPI | - |
dc.subject.keywordAuthor | GSH | - |
dc.subject.keywordAuthor | JC-1 | - |
dc.subject.keywordAuthor | mitochondrial membrane potential | - |
dc.subject.keywordAuthor | MMP | - |
dc.subject.keywordAuthor | N-acetyl-l-cysteine | - |
dc.subject.keywordAuthor | NAC | - |
dc.subject.keywordAuthor | Neutrophils | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | ROS | - |
dc.subject.keywordAuthor | Trichomonas vaginalis | - |
dc.subject.keywordAuthor | z-Asp-Glu-Val-Asp-fmk | - |
dc.subject.keywordAuthor | z-DEVD-fmk | - |
dc.subject.keywordAuthor | z-VAD-fmk | - |
dc.subject.keywordAuthor | z-Val-Ala-Asp-fmk | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0014489407001749?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.