Chronic furosemide or hydrochlorothiazide administration increases H+-ATPase B1 subunit abundance in rat kidney
DC Field | Value | Language |
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dc.contributor.author | Na, Ki Young | - |
dc.contributor.author | Kim, Gheun-Ho | - |
dc.contributor.author | Joo, Kwon Wook | - |
dc.contributor.author | Lee, Jay Wook | - |
dc.contributor.author | Jang, Hye Ryoun | - |
dc.contributor.author | Oh, Yun Kyu | - |
dc.contributor.author | Jeon, Un Sil | - |
dc.contributor.author | Chae, Seoung-Wan | - |
dc.contributor.author | Knepper, Mark A. | - |
dc.contributor.author | Han, Jin Suk | - |
dc.date.accessioned | 2022-10-07T11:27:42Z | - |
dc.date.available | 2022-10-07T11:27:42Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-06 | - |
dc.identifier.issn | 1931-857X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/172294 | - |
dc.description.abstract | Furosemide administration stimulates distal acidification. This has been attributed to the increased lumen-negative voltage in the distal nephron, but the aspect of regulatory mechanisms of H+-ATPase has not been clear. The purpose of this study is to investigate whether chronic administration of diuretics alters the expression of H+-ATPase and whether electrogenic Na+ reabsorption is involved in this process. A 7-day infusion of furosemide or hydrochlorothiazide (HCTZ) lowered urine pH significantly. However, this effect of furosemide-induced distal acidification was not changed with amiloride-blocking electrogenic Na+ reabsorption. On immunoblotting, a polyclonal antibody against the H+-ATPase B1 subunit recognized a specific similar to 56-kDa band in membrane fractions from the kidney. The protein abundance of H+-ATPase was significantly increased by furosemide and HCTZ infusion in both the cortex and outer medulla. Furosemide plus amiloride administration also increased the H+-ATPase protein abundance significantly. However, no definite subcellular redistribution of H+-ATPase was observed by furosemide +/- amiloride infusion with immunohistochemistry. Chronic furosemide +/- amiloride administration induced a translocation of pendrin to the apical membrane, while total protein abundance was not increased. The mRNA expression of H+-ATPase was not altered by furosemide +/- amiloride infusion. We conclude that chronic administration of diuretics enhances distal acidification by increasing the abundance of H+-ATPase irrespective of electrogenic Na+ reabsorption. This upregulation of H+-ATPase in the intercalated cells may be the result of tubular hypertrophy by diuretics. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER PHYSIOLOGICAL SOC | - |
dc.title | Chronic furosemide or hydrochlorothiazide administration increases H+-ATPase B1 subunit abundance in rat kidney | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Gheun-Ho | - |
dc.identifier.doi | 10.1152/ajprenal.00270.2006 | - |
dc.identifier.scopusid | 2-s2.0-34447650614 | - |
dc.identifier.wosid | 000247942000005 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.292, no.6, pp.F1701 - F1709 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | - |
dc.citation.volume | 292 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | F1701 | - |
dc.citation.endPage | F1709 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | RENAL INTERCALATED CELLS | - |
dc.subject.keywordPlus | MOUSE KIDNEY | - |
dc.subject.keywordPlus | URINARY ACIDIFICATION | - |
dc.subject.keywordPlus | COLLECTING DUCT | - |
dc.subject.keywordPlus | PENDRIN | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DIURETICS | - |
dc.subject.keywordPlus | B1-SUBUNIT | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordAuthor | distal acidification | - |
dc.subject.keywordAuthor | amiloride | - |
dc.subject.keywordAuthor | polyclonal anti-H+-ATPase B1 subunit antibody | - |
dc.subject.keywordAuthor | pendrin | - |
dc.subject.keywordAuthor | intercalated cells | - |
dc.identifier.url | https://journals.physiology.org/doi/full/10.1152/ajprenal.00270.2006 | - |
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