Enhanced cellular delivery and transfection efficiency of plasmid DNA using positively charged biocompatible colloidal gold nanoparticles
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Noh, Sang Myoung | - |
dc.contributor.author | Kim, Won-Ki | - |
dc.contributor.author | Kim, Sun Jae | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Baek, Kwang-Hyun | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.date.accessioned | 2022-10-07T11:29:06Z | - |
dc.date.available | 2022-10-07T11:29:06Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.issn | 0304-4165 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/172308 | - |
dc.description.abstract | Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this study, we report an enhancement of the transfection efficiency of plasmid DNA, via the use of positively charged colloidal gold nanoparticles (PGN). Plasmid DNA encoding for murine interleukin-2 (pVAXmIL-2) was complexed with PGN at a variety of ratios. The delivery of pVAXmIL-2 into C2C12 cells was dependent on the complexation ratios between PGN and the plasmid DNA, presented the highest delivery at a ratio of 2400:1. After complexation with DNA, PGN showed significantly higher cellular delivery and transtection efficiency than did the polyethylenimines (PEI) of different molecular weights, such as PEI25K (m.w. 25 kd) and PEI2K (m.w. 2 kd). PGN resulted in a cellular delivery of pVAXmIL-2 6.3-fold higher than was seen with PE125K. The PGN/DNA complex resulted in 3.2- and 2. 1 -fold higher murine IL-2 protein expression than was seen in association with the PEI25K/DNA and PEI2K/DNA complexes, respectively. Following intramuscular administration, PGN/DNA complexes showed more than 4 orders of magnitude higher expression levels as compared to naked DNA. Moreover, the PGN/DNA complexes showed higher cell viability than other cationic nonviral vectors. Collectively, the results of this study suggest that the PGN/DNA complexes may harbor the potential for development into efficient and safe gene delivery vehicles. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | Enhanced cellular delivery and transfection efficiency of plasmid DNA using positively charged biocompatible colloidal gold nanoparticles | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.1016/j.bbagen.2007.01.012 | - |
dc.identifier.scopusid | 2-s2.0-33947187640 | - |
dc.identifier.wosid | 000245832500003 | - |
dc.identifier.bibliographicCitation | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v.1770, no.5, pp.747 - 752 | - |
dc.relation.isPartOf | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | - |
dc.citation.title | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | - |
dc.citation.volume | 1770 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 747 | - |
dc.citation.endPage | 752 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | PLACEBO-CONTROLLED MULTICENTER | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordAuthor | nonviral gene delivery | - |
dc.subject.keywordAuthor | colloidal gold nanoparticle | - |
dc.subject.keywordAuthor | transtection efficiency | - |
dc.subject.keywordAuthor | cationic vector | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0304416507000311?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.