Identification of tyrosine-nitrated proteins in HT22 hippocampal cells during glutamate-induced oxidative stress
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, S-W | - |
dc.contributor.author | Kang, S. | - |
dc.contributor.author | Ryu, S-E | - |
dc.contributor.author | Poo, H. | - |
dc.date.accessioned | 2022-12-20T10:52:53Z | - |
dc.date.available | 2022-12-20T10:52:53Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2010-12 | - |
dc.identifier.issn | 0960-7722 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/173391 | - |
dc.description.abstract | Objectives: Nitration of tyrosine residues in protein is a post-translational modification, which occurs under oxidative stress, and is associated with several neurodegenerative diseases. To understand the role of nitrated proteins in oxidative stress-induced cell death, we identified nitrated proteins and checked correlation of their nitration in glutamate-induced HT22 cell death. Materials and methods: Nitrated proteins were detected by western blotting using an anti-nitrotyrosine antibody, extracted from matching reference 2-dimensional electrophoresis gels, and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: Glutamate treatment induced apoptosis in HT22 cells, while reactive oxygen species (ROS) inhibitor or neuronal nitric oxide synthase (nNOS) inhibitor blocked glutamate-induced HT22 cell death. Nitration levels of 13 proteins were increased after glutamate stimulation; six of them were involved in regulation of energy production and two were related to apoptosis. The other nitrated proteins were associated with calcium signal modulation, ER dysfunction, or were of unknown function. Conclusions: The 13 tyrosine-nitrated proteins were detected in these glutamate-treated HT22 cells. Results demonstrated that cell death, ROS accumulation and nNOS expression were related to nitration of protein tyrosine in the glutamate-stimulated cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Identification of tyrosine-nitrated proteins in HT22 hippocampal cells during glutamate-induced oxidative stress | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ryu, S-E | - |
dc.identifier.doi | 10.1111/j.1365-2184.2010.00708.x | - |
dc.identifier.scopusid | 2-s2.0-78049464342 | - |
dc.identifier.wosid | 000284614300007 | - |
dc.identifier.bibliographicCitation | CELL PROLIFERATION, v.43, no.6, pp.584 - 593 | - |
dc.relation.isPartOf | CELL PROLIFERATION | - |
dc.citation.title | CELL PROLIFERATION | - |
dc.citation.volume | 43 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 584 | - |
dc.citation.endPage | 593 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | PROTEOMIC IDENTIFICATION | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | SUPEROXIDE | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | MODULATION | - |
dc.subject.keywordPlus | CHAPERONE | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2184.2010.00708.x | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.