ASK1 Negatively Regulates the 26 S Proteasome
DC Field | Value | Language |
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dc.contributor.author | Um, Ji Won | - |
dc.contributor.author | Im, Eunju | - |
dc.contributor.author | Park, Joongkyu | - |
dc.contributor.author | Oh, Yohan | - |
dc.contributor.author | Min, Boram | - |
dc.contributor.author | Lee, Hyun Jung | - |
dc.contributor.author | Yoon, Jong Bok | - |
dc.contributor.author | Chung, Kwang Chul | - |
dc.date.accessioned | 2022-12-20T11:23:01Z | - |
dc.date.available | 2022-12-20T11:23:01Z | - |
dc.date.created | 2021-10-15 | - |
dc.date.issued | 2010-11 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/173565 | - |
dc.description.abstract | The 26 S proteasome, composed of the 20 S core and 19 S regulatory particle, plays a central role in ubiquitin-dependent proteolysis. Disruption of this process contributes to the pathogenesis of the various diseases; however, the mechanisms underlying the regulation of 26 S proteasome activity remain elusive. Here, cell culture experiments and in vitro assays demonstrated that apoptosis signal-regulating kinase 1 (ASK1), a member of the MAPK kinase kinase family, negatively regulated 26 S proteasome activity. Immunoprecipitation/Western blot analyses revealed that ASK1 did not interact with 20 S catalytic core but did interact with ATPases making up the 19 S particle, which is responsible for recognizing polyubiquitinated proteins, unfolding them, and translocating them into the 20 S catalytic core in an ATP-dependent process. Importantly, ASK1 phosphorylated Rpt5, an AAA ATPase of the 19 S proteasome, and inhibited its ATPase activity, an effect that may underlie the ability of ASK1 to inhibit 26 S proteasome activity. The current findings point to a novel role for ASK1 in the regulation of 26 S proteasome and offer new strategies for treating human diseases caused by proteasome malfunction. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | ASK1 Negatively Regulates the 26 S Proteasome | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Oh, Yohan | - |
dc.identifier.doi | 10.1074/jbc.M110.133777 | - |
dc.identifier.scopusid | 2-s2.0-78449252451 | - |
dc.identifier.wosid | 000284146100073 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.285, no.47, pp.36434 - 36446 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 285 | - |
dc.citation.number | 47 | - |
dc.citation.startPage | 36434 | - |
dc.citation.endPage | 36446 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | NF-KAPPA-BCASPASE ACTIVATIONFAMILY PROTEINSATPASE SUBUNITCELL-DEATHAPOPTOSISPHOSPHORYLATIONPATHWAYKINASESTRESS | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0021925820467936 | - |
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