Effects of the knockdown of hypoxia inducible factor-1α expression by adenovirus-mediated shRNA on angiogenesis and tumor growth in hepatocellular carcinoma cell linesEffects of the knockdown of hypoxia inducible factor-1α expression by adenovirus-mediated shRNA on angiogenesis and tumor growth in hepatocellular carcinoma cell lines
- Other Titles
- Effects of the knockdown of hypoxia inducible factor-1α expression by adenovirus-mediated shRNA on angiogenesis and tumor growth in hepatocellular carcinoma cell lines
- Authors
- 최성훈; 신혜원; 박준용; 유지영; 김도영; 노원상; 윤채옥; 한광협
- Issue Date
- Sep-2010
- Publisher
- 대한간학회
- Keywords
- Angiogenesis; Hypoxia-inducible factor-1α (HIF-1α); Adenovirus; Small hairpin RNA (shRNA); Human umbilical vein endothelial cells (HUVECs); Hepatocellular carcinoma
- Citation
- Clinical and Molecular Hepatology, v.16, no.3, pp.280 - 287
- Indexed
- KCI
- Journal Title
- Clinical and Molecular Hepatology
- Volume
- 16
- Number
- 3
- Start Page
- 280
- End Page
- 287
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/173699
- DOI
- 10.3350/kjhep.2010.16.3.29
- ISSN
- 2287-2728
- Abstract
- Background/Aims: Hypoxia-inducible factor-1α (HIF-1α) is a central transcriptional factor involved in the cellular responses related to various aspects of cancer biology, including proliferation, survival, and angiogenesis, and the metabolism of the extracellular matrix in hypoxia. This study evaluated whether adenovirus-mediated small hairpin RNA (shRNA) against HIF-1α (shHIF-1α) inhibits cell proliferation and angiogenesis in hepatocellular carcinoma (HCC) cell lines. Methods: Knockdown of HIF-1α expression was constructed by adenovirus-mediated RNA interference tools, and HCC cell lines infected with shHIF-1α coding virus were cultured under a hypoxia condition (1% O2) for 24 hours. Following infection, the expression levels of HIF-1α, angiogenesis factors, and matrix metalloproteinase (MMP) were examined using Western blotting. Cell proliferation and angiogenesis were measured by a cell proliferation assay (MTT assay) and an angiogenesis-related assay (invasion and tube-formation assay), respectively. Results: Adenovirus mediated inhibition of HIF-1α induced suppression of tumor growth in HCC cell lines. It also down-regulated the expression of angiogenesis factor and MMP proteins. Angiogenesis as well as mobility of vascular cells to tumor was suppressed by adenovirus-mediated shHIF-1α-infected groups in human umbilical vein endothelial cells (HUVECs). Conclusions: These data suggest that adenovirus-mediated inhibition of HIF-1αinhibits the invasion, tube formation, and cell growth in HUVECs and HCC cells.
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