miR-124a Is Important for Migratory Cell Fate Transition During Gastrulation of Human Embryonic Stem Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Man Ryul | - |
dc.contributor.author | Kim, Jong Soo | - |
dc.contributor.author | Kim, Kye-Seong | - |
dc.date.accessioned | 2022-12-20T15:49:21Z | - |
dc.date.available | 2022-12-20T15:49:21Z | - |
dc.date.created | 2022-08-27 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174150 | - |
dc.description.abstract | Precise control of gene expression is of paramount importance for proper embryonic development. Although a number of microRNAs (miRNAs) has been implicated in fine-tuning mRNA translation during development, their exact roles for gastrulation, particularly in connection with functional targets, have yet to be clarified, with regard to stage-specific cell migration to form three embryonic germ layers. We found that miR-124a is expressed in human embryonic stem cells (hESC), but is gradually downregulated during embryoid body (EB) formation in vitro. We also provide evidence that SLUG and IQGAP1, which modulates rearrangement of the migratory cytoskeleton, are specific targets for miR-124a during EB forma-tion. Furthermore, we show that the beginning of cell migration, a hallmark event in gastrulation, is tightly coupled with downregulation of miR-124a during EB formation and induction of SLUG and IQGAP1. Overexpressed miR-124a in hESC reduced expression of SLUG and IQGAP1 and blocked migratory cell behavior in EB. An expression level of MIXL1, associated with gastulation process, was also inversely correlated with expression of miR-124a. Taken together, our results strongly suggest that miR-124a may play an active role in inhibiting hESCs from differentiation into EB by downregulating expression of SLUG and IQGAP1, thereby maintaining stemness. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | miR-124a Is Important for Migratory Cell Fate Transition During Gastrulation of Human Embryonic Stem Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kye-Seong | - |
dc.identifier.doi | 10.1002/stem.490 | - |
dc.identifier.scopusid | 2-s2.0-77958117982 | - |
dc.identifier.wosid | 000282454700011 | - |
dc.identifier.bibliographicCitation | STEM CELLS, v.28, no.9, pp.1550 - 1559 | - |
dc.relation.isPartOf | STEM CELLS | - |
dc.citation.title | STEM CELLS | - |
dc.citation.volume | 28 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1550 | - |
dc.citation.endPage | 1559 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.subject.keywordPlus | PRIMITIVE STREAK | - |
dc.subject.keywordPlus | MICRORNAS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | SIGNALS | - |
dc.subject.keywordPlus | XENOPUS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | SET | - |
dc.subject.keywordAuthor | Human embryonic stemcells | - |
dc.subject.keywordAuthor | microRNA | - |
dc.subject.keywordAuthor | Cell migration | - |
dc.subject.keywordAuthor | SLUG | - |
dc.subject.keywordAuthor | IQGAP1 | - |
dc.identifier.url | https://academic.oup.com/stmcls/article/28/9/1550/6400997 | - |
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