Control of specific growth rate to enhance the production of a novel disintegrin, saxatilin, in recombinant Pichia pastoris
DC Field | Value | Language |
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dc.contributor.author | Min, Cheol-Ki | - |
dc.contributor.author | Lee, Jin-Won | - |
dc.contributor.author | Chung, Kwang-Hoe | - |
dc.contributor.author | Park, Hong-Woo | - |
dc.date.accessioned | 2022-12-20T15:52:02Z | - |
dc.date.available | 2022-12-20T15:52:02Z | - |
dc.date.created | 2022-08-27 | - |
dc.date.issued | 2010-09 | - |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174177 | - |
dc.description.abstract | The methylotrophic yeast Pichia pastoris is one of the best hosts for the production of foreign proteins because of the presence of a strong alcohol oxidase 1 (AOX1) promoter that can be induced by methanol. Feeding the yeast, methanol induces protein production and provides an energy source for the host cells. However, excessive levels of methanol inhibit the growth of host cells, and insufficient methanol levels lead to poor growth and protein production. We have used various methanol feeding strategies to enhance the production of saxatilin. Saxatilin is a novel snake venom-derived disintegrin that inhibits tumor angiogenesis and metastasis and has been shown to suppress ovarian cancer cell invasion. A two-step increase feeding strategy to control the specific growth rate led to the best results in terms of specific protein production rates and final saxatilin amounts within the limited fermentation time. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SOC BIOSCIENCE BIOENGINEERING JAPAN | - |
dc.title | Control of specific growth rate to enhance the production of a novel disintegrin, saxatilin, in recombinant Pichia pastoris | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Hong-Woo | - |
dc.identifier.doi | 10.1016/j.jbiosc.2010.03.013 | - |
dc.identifier.scopusid | 2-s2.0-77955337355 | - |
dc.identifier.wosid | 000282927300010 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v.110, no.3, pp.314 - 319 | - |
dc.relation.isPartOf | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.citation.title | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.citation.volume | 110 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 314 | - |
dc.citation.endPage | 319 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.subject.keywordPlus | SNAKE GLOYDIUS-SAXATILIS | - |
dc.subject.keywordPlus | FED-BATCH FERMENTATION | - |
dc.subject.keywordPlus | CANCER CELL-LINE | - |
dc.subject.keywordPlus | METHANOL CONCENTRATION | - |
dc.subject.keywordPlus | ANTIBODY FRAGMENT | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | YEAST | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordAuthor | Methylotrophic yeast | - |
dc.subject.keywordAuthor | Pichia pastoris | - |
dc.subject.keywordAuthor | Saxatilin | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1389172310001143?via%3Dihub | - |
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