Transduction of human EPO into human bone marrow mesenchymal stromal cells synergistically enhances cell-protective and migratory effects
DC Field | Value | Language |
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dc.contributor.author | Kim, Mi-Hwa | - |
dc.contributor.author | Cho, Goang-Won | - |
dc.contributor.author | Koh, Seong-Ho | - |
dc.contributor.author | Huh, Yong-Min | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.date.accessioned | 2022-12-20T16:21:08Z | - |
dc.date.available | 2022-12-20T16:21:08Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2010-08 | - |
dc.identifier.issn | 0026-8933 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174400 | - |
dc.description.abstract | Human bone marrow mesenchymal stromal cells (hBM-MSCs) are a promising tools for cell therapy. However, the poor viability of the transplanted cells is a major limiting factor. Human erythropoietin (hEPO) has been extensively studied in non-hematopoietic tissues for its neurotrophic, anti-oxidant, antiapoptotic, and anti-inflammatory effects. In this study, we evaluate whether transduction of the hEPO gene into MSCs provides protection and affects their migration. hBM-MSCs transduced with the hEPO gene (EPO-MSCs) stably secreted high levels of hEPO (10 IU/ml) with no alteration of their mesenchymal phenotype. MSCs were also treated with 10 IU rhEPO, an amount similar to what was secreted by EPO-MSCs, to generate 10U-MSCs. Protection against H2O2-induced oxidative stress and staurosporine-induced apoptosis was registered for both EPO-MSCs and 10U-MSCs, but the protective effects were higher for the EPO-MSCs than for the 10U-MSCs. EPO-MSCs had significantly higher migration rates compared to MSCs and 10U-MSCs. We confirmed that the intracellular signaling of ERK1/2 was higher in the EPO-MSCs than 10U-MSCs. This data demonstrates that the endogenous expression of EPO may efficiently initiate the ERK1/2 signaling pathway, resulting in synergistic effects on the production of neurotrophic factors. Thus, EPO-MSCs are a good candidate for cell therapy in ischemic and neurodegenerative diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MAIK NAUKA/INTERPERIODICA/SPRINGER | - |
dc.title | Transduction of human EPO into human bone marrow mesenchymal stromal cells synergistically enhances cell-protective and migratory effects | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Koh, Seong-Ho | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hyun | - |
dc.identifier.doi | 10.1134/S0026893310040126 | - |
dc.identifier.scopusid | 2-s2.0-77955355844 | - |
dc.identifier.wosid | 000280702100012 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOLOGY, v.44, no.4, pp.577 - 584 | - |
dc.relation.isPartOf | MOLECULAR BIOLOGY | - |
dc.citation.title | MOLECULAR BIOLOGY | - |
dc.citation.volume | 44 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 577 | - |
dc.citation.endPage | 584 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | CEREBRAL-ISCHEMIA MODEL | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | NEUROTROPHIC FACTOR | - |
dc.subject.keywordPlus | ADULT-RAT | - |
dc.subject.keywordPlus | ERYTHROPOIETIN | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | DIFFERENTIATE | - |
dc.subject.keywordAuthor | bone marrow mesenchymal stromal cells | - |
dc.subject.keywordAuthor | erythropoietin | - |
dc.subject.keywordAuthor | cell protection | - |
dc.identifier.url | https://link.springer.com/article/10.1134/S0026893310040126 | - |
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