Effects of Endocrine Disruptors on Bombina orientalis P450 Aromatase Activity
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyung-min | - |
dc.contributor.author | Yang, Wonseok | - |
dc.contributor.author | Rhee, Jae-Sung | - |
dc.contributor.author | Hwang, Dae-Sik | - |
dc.contributor.author | Park, Chan Jin | - |
dc.contributor.author | Gye, Myung Chan | - |
dc.contributor.author | Lee, Jae-Seong | - |
dc.contributor.author | Shin, Incheol | - |
dc.date.accessioned | 2022-12-20T18:12:17Z | - |
dc.date.available | 2022-12-20T18:12:17Z | - |
dc.date.created | 2022-08-27 | - |
dc.date.issued | 2010-04 | - |
dc.identifier.issn | 0289-0003 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/175155 | - |
dc.description.abstract | To assess the effects of endocrine-disrupting chemicals on the expression and activity of aromatase in the gonads of Bombina orientalis, a common amphibian, we intraperitoneally injected nonylphenol or bisphenol-A and then examined aromatase mRNA levels by RT-PCR as well as aromatase enzymatic activity by tritiated water release assays. To design primers for the RT-PCR, we cloned the B. orientalis aromatase gene using RT-PCR and degenerate primers. The full-length cDNA was obtained by 5'- and 3'-RACE PCR. The complete sequence of the B. orientalis aromatase gene revealed an open reading frame of 1500 bp encoding a deduced protein of 500 amino acids. Semi-quantitative RT-PCR indicated that nonylphenol or bisphenol-A injection did not significantly affect the expression of B. orientalis aromatase mRNA. However, a 48-hr treatment with nonyphenol or bisphenol A reduced aromatase activity to 47% and 32% of the control, respectively. These results suggest that endocrine disruptors can effectively modulate the activity of B. orientalis aromatase without affecting its mRNA levels. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ZOOLOGICAL SOC JAPAN | - |
dc.title | Effects of Endocrine Disruptors on Bombina orientalis P450 Aromatase Activity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gye, Myung Chan | - |
dc.contributor.affiliatedAuthor | Shin, Incheol | - |
dc.identifier.doi | 10.2108/zsj.27.338 | - |
dc.identifier.scopusid | 2-s2.0-77950991743 | - |
dc.identifier.wosid | 000276175000006 | - |
dc.identifier.bibliographicCitation | ZOOLOGICAL SCIENCE, v.27, no.4, pp.338 - 343 | - |
dc.relation.isPartOf | ZOOLOGICAL SCIENCE | - |
dc.citation.title | ZOOLOGICAL SCIENCE | - |
dc.citation.volume | 27 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 338 | - |
dc.citation.endPage | 343 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Zoology | - |
dc.relation.journalWebOfScienceCategory | Zoology | - |
dc.subject.keywordPlus | TILAPIA OREOCHROMIS-NILOTICUS | - |
dc.subject.keywordPlus | VITELLOGENIN MESSENGER-RNA | - |
dc.subject.keywordPlus | NEWT PLEURODELES-WALTL | - |
dc.subject.keywordPlus | BISPHENOL-A | - |
dc.subject.keywordPlus | SEX-REVERSAL | - |
dc.subject.keywordPlus | CYTOCHROME-P450 AROMATASE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CYP19 GENE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordAuthor | amphibians | - |
dc.subject.keywordAuthor | aromatase | - |
dc.subject.keywordAuthor | bisphenol-A | - |
dc.subject.keywordAuthor | steroid | - |
dc.subject.keywordAuthor | steroidogenesis | - |
dc.identifier.url | https://bioone.org/journals/zoological-science/volume-27/issue-4/zsj.27.338/Effects-of-Endocrine-Disruptors-on-Bombina-orientalis-P450-Aromatase-Activity/10.2108/zsj.27.338.short | - |
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