A guanidinylated bioreducible polymer with high nuclear localization ability for gene delivery systems
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae-il | - |
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.date.accessioned | 2022-12-20T18:47:49Z | - |
dc.date.available | 2022-12-20T18:47:49Z | - |
dc.date.created | 2022-08-27 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/175364 | - |
dc.description.abstract | Guanidinylated bioreducible polymer (GBP) was developed for gene delivery systems utilizing cellular penetrating ability of guanidine groups. GBP could retard pDNA from a weight ratio of 5 completely in agarose gel electrophoresis but pDNA was released from GBP polyplexes even at a weight ratio of 20 in reducing condition (2.5 mm DTT) due to their biodegradation. GBP also could construct 200 nm-sized and positively charged (similar to 30 mV) polyplex nanoparticles with pDNA. The cytotoxicity of GBP was found to be minimal and GBP showed about 8 folds improved transfection efficiency than a scaffold polymer, poly(cystaminebisacrylamide-diaminohexane) (poly(CBA-DAH)) and even higher transfection efficiency than PEI25k in mammalian cell lines. Its high cellular uptake efficiency (96.1%) and strong nuclear localization ability for pDNA delivery due to the structural advantage of bioreducible polymer and guanidine groups were also identified, suggesting GBP is a promising candidate for efficient gene delivery systems. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | A guanidinylated bioreducible polymer with high nuclear localization ability for gene delivery systems | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.biomaterials.2009.10.034 | - |
dc.identifier.scopusid | 2-s2.0-73249136229 | - |
dc.identifier.wosid | 000274354400037 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.31, no.7, pp.1798 - 1804 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 31 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1798 | - |
dc.citation.endPage | 1804 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | PLASMID DNA DELIVERY | - |
dc.subject.keywordPlus | POLY(DISULFIDE AMINE) | - |
dc.subject.keywordPlus | POLY(AMIDO AMINE)S | - |
dc.subject.keywordPlus | CARGO DELIVERY | - |
dc.subject.keywordPlus | TAT PEPTIDE | - |
dc.subject.keywordPlus | MICROPARTICLES | - |
dc.subject.keywordPlus | TRANSPORTERS | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | CARRIERS | - |
dc.subject.keywordAuthor | Bioreducible polymer | - |
dc.subject.keywordAuthor | Gene transfer | - |
dc.subject.keywordAuthor | Guanidinylation | - |
dc.subject.keywordAuthor | Cytotoxicity | - |
dc.subject.keywordAuthor | Nuclear localization ability | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0142961209011211?via%3Dihub | - |
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