Conditions for Tumor-free and Dopamine Neuron-enriched Grafts After Transplanting Human ES Cell-derived Neural Precursor Cells
DC Field | Value | Language |
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dc.contributor.author | Ko, Ji-Yun | - |
dc.contributor.author | Lee, Hyun-Seob | - |
dc.contributor.author | Park, Chang-Hwan | - |
dc.contributor.author | Koh, Hyun-Chul | - |
dc.contributor.author | Lee, Yong-Sung | - |
dc.contributor.author | Lee, Sang-Hun | - |
dc.date.accessioned | 2022-12-20T20:34:13Z | - |
dc.date.available | 2022-12-20T20:34:13Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-10 | - |
dc.identifier.issn | 1525-0016 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176077 | - |
dc.description.abstract | We have previously demonstrated derivation of neural precursor (NP) cells of a midbrain-type from human embryonic stem (hES) cells to yield an enriched population of dopamine (DA) neurons. These hES-derived NPs can be expanded in vitro through multiple passages without altering their DA neurogenic potential. Here, we studied two aspects of these hES-NP cells that are critical issues in cell therapeutic approaches for Parkinson's disease (PD): cell survival and tumorigenic potential. Neuroepithelial rosettes, a potentially tumorigenic structure, disappeared during hES-NP cell expansion in vitro. Although a minor population of cells positive for Oct3/4, a marker specific for undifferentiated hES cells, persisted in culture during hES-NP cell expansion, they could be completely eliminated by subculturing hES-NPs under differentiation-inducing conditions. Consistently, no tumors/teratomas are formed in rats grafted with multipassaged hES-NPs. However, extensively expanded hES-NP cells easily underwent cell death during differentiation in vitro and after transplantation in vivo. Transgenic expression of Bcl-XL and sonic hedgehog (SHH) completely overcame the cell survival problems without increasing tumor formation. These findings indicate that hES-NP cell expansion in conjunction with Bcl-XL+ SHH transgene expression may provide a renewable and safe source of DA neurons for transplantation in PD. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | CELL PRESS | - |
dc.title | Conditions for Tumor-free and Dopamine Neuron-enriched Grafts After Transplanting Human ES Cell-derived Neural Precursor Cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Chang-Hwan | - |
dc.contributor.affiliatedAuthor | Koh, Hyun-Chul | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hun | - |
dc.identifier.doi | 10.1038/mt.2009.148 | - |
dc.identifier.scopusid | 2-s2.0-70349880527 | - |
dc.identifier.wosid | 000270851900014 | - |
dc.identifier.bibliographicCitation | MOLECULAR THERAPY, v.17, no.10, pp.1761 - 1770 | - |
dc.relation.isPartOf | MOLECULAR THERAPY | - |
dc.citation.title | MOLECULAR THERAPY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1761 | - |
dc.citation.endPage | 1770 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | BCL-X-L | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | TISSUE-SECTIONS | - |
dc.subject.keywordPlus | SONIC HEDGEHOG | - |
dc.subject.keywordPlus | NEUROTROPHIC FACTOR | - |
dc.subject.keywordPlus | ANTIGEN RETRIEVAL | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | MIDBRAIN | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1525001616321402?via%3Dihub | - |
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