Genistein enhances TRAIL-induced apoptosis through inhibition of p38 MAPK signaling in human hepatocellular carcinoma Hep3B cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jin, Cheng-Yun | - |
dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Kim, Gi-Young | - |
dc.contributor.author | Lee, Su-Jae | - |
dc.contributor.author | Kim, Wun-Jae | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.accessioned | 2022-12-20T21:45:54Z | - |
dc.date.available | 2022-12-20T21:45:54Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-07 | - |
dc.identifier.issn | 0009-2797 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176592 | - |
dc.description.abstract | The cytotoxic effect of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is limited in some carcinoma cancer cells. However, it was found that treatment with TRAIL in combination with nontoxic concentrations of genistein sensitized TRAIL-resistant human hepatocellular carcinoma Hep3B cells to TRAIL-mediated apoptosis. Combined treatment with genistein and TRAIL-induced chromatin condensation and sub-G1 phase DNA content. These indicators of apoptosis were correlated with the induction of caspase activity that resulted in the cleavage of poly(ADP-ribose) polymerase (PARP). Both cell viability and the cleavage of PARP induced by combined treatment were significantly inhibited by caspase-3, -8 and -9 inhibitors, which demonstrates the important roles of caspases in the observed cytotoxic effects. Genistein treatment also triggered the inhibition of p38-beta mitogen-activated protein kinase (MAPK) activation. Pretreatment with SB203580 resulted in significantly increased sub-G1 population and loss of mitochondrial membrane potential (MMP) in TRAIL-induced apoptosis. By contrast, overexpression of p38 MAPK protected apoptosis by co-treatment with genistein and TRAIL, suggesting that the p38 MAPK act as key regulators of apoptosis in response to treatment with a combination of genistein and TRAIL in human hepatocellular carcinoma Hep3B cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Genistein enhances TRAIL-induced apoptosis through inhibition of p38 MAPK signaling in human hepatocellular carcinoma Hep3B cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Su-Jae | - |
dc.identifier.doi | 10.1016/j.cbi.2009.03.020 | - |
dc.identifier.scopusid | 2-s2.0-67349246450 | - |
dc.identifier.wosid | 000267584000003 | - |
dc.identifier.bibliographicCitation | CHEMICO-BIOLOGICAL INTERACTIONS, v.180, no.2, pp.143 - 150 | - |
dc.relation.isPartOf | CHEMICO-BIOLOGICAL INTERACTIONS | - |
dc.citation.title | CHEMICO-BIOLOGICAL INTERACTIONS | - |
dc.citation.volume | 180 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 143 | - |
dc.citation.endPage | 150 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | MEDIATED UP-REGULATION | - |
dc.subject.keywordPlus | PROSTATE-CANCER CELLS | - |
dc.subject.keywordPlus | ANTITUMOR-ACTIVITY | - |
dc.subject.keywordPlus | TYROSINE-KINASES | - |
dc.subject.keywordPlus | G2/M ARREST | - |
dc.subject.keywordPlus | CYCLIN B1 | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordAuthor | Genistein | - |
dc.subject.keywordAuthor | TRAIL | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | p38 MAPK | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0009279709001379?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.