Dexamethasone-conjugated polyethylenimine as an efficient gene carrier with an anti-apoptotic effect to cardiomyocytes
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyunjung | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Bae, Yun Mi | - |
dc.contributor.author | Choi, Joon Sig | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-12-20T21:56:04Z | - |
dc.date.available | 2022-12-20T21:56:04Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-06 | - |
dc.identifier.issn | 1099-498X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176691 | - |
dc.description.abstract | Background Dexamethasone is a potent glucocorticoid with anti-inflammatory effects. Dexamethasone can protect ischemic cardiomyocytes from apoptosis. To apply the anti-apoptotic effect of dexamethasone to ischemic disease gene therapy, dexamethasone-conjugated polyethylenimine (PEI-Dexa) was synthesized and evaluated as an anti-apoptotic gene carrier. Methods PEI-Dexa was synthesized with low molecular weight polyethylenimine (PEI2K, 2 kDa). The transfection efficiency and cytotoxicity of PEI-Dexa were evaluated by luciferase assay and the MTT assay. To evaluate the anti-apoptotic effect, PEI-Dexa/DNA complex was transfected into cells and the cells were treated with H2O2. Cell viability and apoptosis level were measured by the MTT assay and caspase-3 assay, respectively. Results A transfection assay into H9C2 rat cardiomyocytes showed that PEI-Dexa had the highest transfection efficiency at an 8 : 1 weight ratio (PEI-Dexa/DNA). At this ratio, PEI-Dexa had higher transfection efficiency than high molecular polyethylenimine (PEI25K, 25 kDa) and PEI2K. In addition, the cytotoxicity of PEI-Dexa was lower than that of PEI25K. To evaluate the anti-apoptotic effect, PEI-Dexa/pSV-Luc or PEI2K/pSV-Luc was transfected into H9C2 cells and the cells were treated with H2O2. PEI-Dexa was found to reduce caspase-3 activity and increase cell viability compared to PEI2K. Heme oxygenase-1 (HO-1) can protect ischemic cardiomyocytes from apoptosis. Therefore, pSV-HO-1 was cloned and transfected into H9C2 cells using PEI-Dexa. The cells transfected with PEI-Dexa/pSV-HO-1 complex had lower caspase-3 activity and higher viability than the cells transfected with PEI-Dexa/pSV-Luc complex after the H2O2 treatment. Conclusions PEI-Dexa is an efficient gene carrier with an anti-apoptotic effect and may be useful for anti-apoptotic gene therapy in combination with pSV-HO-1. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Dexamethasone-conjugated polyethylenimine as an efficient gene carrier with an anti-apoptotic effect to cardiomyocytes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunjung | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1002/jgm.1320 | - |
dc.identifier.scopusid | 2-s2.0-65949101881 | - |
dc.identifier.wosid | 000267079700007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GENE MEDICINE, v.11, no.6, pp.515 - 522 | - |
dc.relation.isPartOf | JOURNAL OF GENE MEDICINE | - |
dc.citation.title | JOURNAL OF GENE MEDICINE | - |
dc.citation.volume | 11 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 515 | - |
dc.citation.endPage | 522 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | CARDIAC MYOCYTE APOPTOSIS | - |
dc.subject.keywordPlus | WATER-SOLUBLE LIPOPOLYMER | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | DNA DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | HYPERTROPHY | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | DOXORUBICIN | - |
dc.subject.keywordPlus | MYOCARDIUM | - |
dc.subject.keywordAuthor | dexamethasone | - |
dc.subject.keywordAuthor | gene carrier | - |
dc.subject.keywordAuthor | heme oxygenase-1 | - |
dc.subject.keywordAuthor | hydrogen peroxide | - |
dc.subject.keywordAuthor | polyethylenimine | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/jgm.1320 | - |
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