Enhanced protection of Ins-1 beta cells from apoptosis under hypoxia by delivery of DNA encoding secretion signal peptide-linked exendin-4
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Lee, Suyeon | - |
dc.contributor.author | Park, Jeong-Hyun | - |
dc.contributor.author | Lee, Sanghyun | - |
dc.contributor.author | Lee, Byung-Wan | - |
dc.contributor.author | Ihm, Sung Hee | - |
dc.contributor.author | Kim, Tae-il | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.contributor.author | Ko, Kyung Soo | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-12-20T22:45:11Z | - |
dc.date.available | 2022-12-20T22:45:11Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176996 | - |
dc.description.abstract | In this study, we developed an expression system of exendin-4, a glucagon-like peptide (GLP-1) analog, using a secretion signal peptide (SP) to facilitate exendin-4 secretion. For delivery of the exendin-4 expression system, high-molecular-weight polyethylenimine (25 kDa, PEI25k), low-molecular-weight polyethylenimine (2 kDa, PEI2k), and polyamidoamine (PAMAM) dendrimers were evaluated as gene carriers to Ins-1 beta cells. As a result, PEI25k showed the highest transfection efficiency. For the construction of the exendin-4 expression vector, DNA coding the SP sequence was inserted upstream of the exendin-4 cDNA, resulting in the construction of p beta-SP-Ex-4. Transfection assay showed that the secretion level of exendin-4 increased in the p beta-SP-Ex-4 transfected cells, compared with the p beta-Ex-4 transfected cells. To identify the beta-cell protection effect of p beta-SP-Ex-4 delivery, the Ins-1 beta cells were transfected with p beta-SP-Ex-4 or p beta-Ex-4 and incubated under normoxia or hypoxia. An MTT assay showed that the p beta-SP-Ex-4 transfected cells had higher beta-cell viability than the p beta-Ex-4 transfected cells under hypoxia. In addition, the p beta-SP-Ex-4 transfected cells exhibited lower caspase-3 activity than the p beta-Ex-4 transfected cells. Therefore, PEI25k/p beta-SP-Ex-4 complex may be useful to protect isolated beta cells from apoptosis during transplantation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Enhanced protection of Ins-1 beta cells from apoptosis under hypoxia by delivery of DNA encoding secretion signal peptide-linked exendin-4 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1080/10611860902718664 | - |
dc.identifier.scopusid | 2-s2.0-70149118782 | - |
dc.identifier.wosid | 000266593400008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.17, no.3, pp.242 - 248 | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 17 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 242 | - |
dc.citation.endPage | 248 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | GLUCAGON-LIKE PEPTIDE-1 | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | TYPE-2 DIABETIC-PATIENTS | - |
dc.subject.keywordPlus | HUMAN PANCREATIC-ISLETS | - |
dc.subject.keywordPlus | FACTOR GENE DELIVERY | - |
dc.subject.keywordPlus | BCL-2 GENE | - |
dc.subject.keywordPlus | VEGF GENE | - |
dc.subject.keywordPlus | RAT ISLETS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | GLP-1 | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | cytoprotection | - |
dc.subject.keywordAuthor | exendin-4 | - |
dc.subject.keywordAuthor | glucagon-like peptide-1 | - |
dc.subject.keywordAuthor | hypoxia | - |
dc.subject.keywordAuthor | signal peptide | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/10611860902718664 | - |
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