STAT3 is involved in phosphatidic acid-induced Bcl-2 expression in HeLa cells
DC Field | Value | Language |
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dc.contributor.author | Choi, Hye-Jin | - |
dc.contributor.author | Lee, Jung Han | - |
dc.contributor.author | Park, Shin-Young | - |
dc.contributor.author | Cho, Ju Hwan | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.date.accessioned | 2022-12-20T23:41:07Z | - |
dc.date.available | 2022-12-20T23:41:07Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-02 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177342 | - |
dc.description.abstract | Phosphatidic acid (PA), the product of a PLD-mediated reaction, is a lipid second messenger that participates in various intracellular signaling events and is known to regulate a growing list of signaling proteins. We found that Bcl-2 was upregulated by PA treatment in HeLa cells. However, how PA upregulates Bcl-2 expression has not yet been studied. In this study, we tried to discover the mechanisms of Bcl-2 up-regulation by PA treatment in HeLa cells. Treatment with PA resulted in significantly increased expression of Bcl-2 in HeLa cells. Moreover, PA-induced Bcl-2 expression was blocked by mepacrine, an inhibitor of PLA(2), but not by propranolol, an inhibitor of PA phospholyhydrolase (PAP). Treatment of 1,2-dipalmitoryl-sn-glycero-3-phosphate (DPPA) also increased Bcl-2 expression. These results indicate that Bcl-2 expression is mediated by lysophosphatidic acid (LPA), not by arachidonic acid (AA). Thereafter, we used MEK1/2 inhibitor, PD98059 to investigate the relationship between ERK1/2 MAPK and PA-induced Bcl-2 expression. PA-induced Bcl-2 expression was decreased when ERK1/2 was inhibited by PD98059. The transcription factor such as STAT3 which is controlled by ERK1/2 MAPK was increased along with Bcl-2 expression when the cells were treated with PA. Furthermore, STAT3 siRNA treatments inhibited PA-induced Bcl-2 expression, suggesting that STAT3 (Ser(727)) is involved in PA-induced Bcl-2 expression. Taken together, these findings indicate that PA acts as an important mediator for increasing Bcl-2 expression through STAT3 (Ser(727)) activation via the ERK1/2 MAPK pathway. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGERNATURE | - |
dc.title | STAT3 is involved in phosphatidic acid-induced Bcl-2 expression in HeLa cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong-Soo | - |
dc.identifier.doi | 10.3858/emm.2009.41.2.012 | - |
dc.identifier.scopusid | 2-s2.0-63849342104 | - |
dc.identifier.wosid | 000263896200005 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.41, no.2, pp.94 - 101 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 41 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 94 | - |
dc.citation.endPage | 101 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001318534 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | SERINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | CANCER CELLS | - |
dc.subject.keywordPlus | TRANSCRIPTIONAL ACTIVITY | - |
dc.subject.keywordPlus | MAXIMAL ACTIVATION | - |
dc.subject.keywordPlus | PROMOTES SURVIVAL | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | PROTEIN FAMILY | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordAuthor | extracellular signal-regulated MAP kinases | - |
dc.subject.keywordAuthor | phosphatidic acids | - |
dc.subject.keywordAuthor | phospholipases A2 | - |
dc.subject.keywordAuthor | proto-oncogene proteins c-bcl-2 | - |
dc.subject.keywordAuthor | STAT3 transcription factor | - |
dc.identifier.url | https://www.nature.com/articles/emm200913 | - |
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