Cell-Penetrating Function of the Poly(ADP-Ribose) (PAR)-Binding Motif Derived from the PAR-Dependent E3 Ubiquitin Ligase Iduna
DC Field | Value | Language |
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dc.contributor.author | Koo, Ja-Hyun | - |
dc.contributor.author | Yoon, Heeseok | - |
dc.contributor.author | Kim, Won-Ju | - |
dc.contributor.author | Cha, Donghun | - |
dc.contributor.author | Choi, Je-Min | - |
dc.date.accessioned | 2021-08-02T13:52:20Z | - |
dc.date.available | 2021-08-02T13:52:20Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/17734 | - |
dc.description.abstract | Iduna is a poly(ADP-ribose) (PAR)-dependent E3 ubiquitin ligase that regulates cellular responses such as proteasomal degradation and DNA repair upon interaction with its substrate. We identified a highly cationic region within the PAR-binding motif of Iduna; the region was similar among various species and showed amino acid sequence similarity with that of known cell-penetrating peptides (CPPs). We hypothesized that this Iduna-derived cationic sequence-rich peptide (Iduna) could penetrate the cell membrane and deliver macromolecules into cells. To test this hypothesis, we generated recombinant Iduna-conjugated enhanced green fluorescent protein (Iduna-EGFP) and its tandem-repeat form (d-Iduna-EGFP). Both Iduna-EGFP and d-Iduna-EGFP efficiently penetrated Jurkat cells, with the fluorescence signals increasing dose- and time-dependently. Tandem-repeats of Iduna and other CPPs enhanced intracellular protein delivery efficiency. The delivery mechanism involves lipid-raft-mediated endocytosis following heparan sulfate interaction; d-Iduna-EGFP was localized in the nucleus as well as the cytoplasm, and its residence time was much longer than that of other controls such as TAT and Hph-1. Moreover, following intravenous administration to C57/BL6 mice, d-Iduna-EGFP was efficiently taken up by various tissues, including the liver, spleen, and intestine suggesting that the cell-penetrating function of the human Iduna-derived peptide can be utilized for experimental and therapeutic delivery of macromolecules. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Cell-Penetrating Function of the Poly(ADP-Ribose) (PAR)-Binding Motif Derived from the PAR-Dependent E3 Ubiquitin Ligase Iduna | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Je-Min | - |
dc.identifier.doi | 10.3390/ijms19030779 | - |
dc.identifier.scopusid | 2-s2.0-85043574064 | - |
dc.identifier.wosid | 000428309800136 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.3 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 19 | - |
dc.citation.number | 3 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | ALLERGIC AIRWAY INFLAMMATION | - |
dc.subject.keywordPlus | HUMAN IMMUNODEFICIENCY VIRUS | - |
dc.subject.keywordPlus | ARGININE-RICH PEPTIDES | - |
dc.subject.keywordPlus | STRAND BREAK REPAIR | - |
dc.subject.keywordPlus | HEPARAN-SULFATE | - |
dc.subject.keywordPlus | ANTENNAPEDIA HOMEODOMAIN | - |
dc.subject.keywordPlus | PROTEIN TRANSDUCTION | - |
dc.subject.keywordPlus | CYTOPLASMIC DOMAIN | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | DNA-DAMAGE | - |
dc.subject.keywordAuthor | Iduna | - |
dc.subject.keywordAuthor | cell-penetrating peptide | - |
dc.subject.keywordAuthor | PAR binding motif | - |
dc.identifier.url | https://www.mdpi.com/1422-0067/19/3/779 | - |
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