Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase
DC Field | Value | Language |
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dc.contributor.author | Nguyen, Duc Long | - |
dc.contributor.author | Kim, Hanim | - |
dc.contributor.author | Kim, Dasom | - |
dc.contributor.author | Lee, Jin Oh | - |
dc.contributor.author | Gye, Myung Chan | - |
dc.contributor.author | Kim, Young-Pil | - |
dc.date.accessioned | 2021-08-02T13:52:22Z | - |
dc.date.available | 2021-08-02T13:52:22Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 1424-8220 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/17736 | - |
dc.description.abstract | We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing process, resulting in a strong association with high bioluminescence signal onto a NeutrAvidin-coated surface. When the peptide substrate for MMP-7 was inserted into a region between luciferase and intein, the biotinylated probe detected MMP-7 activity by cleaving the peptide, and surface-induced bioluminescence signal was strongly reduced in the MMP-secreted media or mouse tissue extracts, compared with that in MMP-deficient control set. Our approach is anticipated to be useful for generating biotinylated proteins and for their applications in diagnosing MMP activity in human diseases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gye, Myung Chan | - |
dc.contributor.affiliatedAuthor | Kim, Young-Pil | - |
dc.identifier.doi | 10.3390/s18030875 | - |
dc.identifier.scopusid | 2-s2.0-85044323317 | - |
dc.identifier.wosid | 000428805300196 | - |
dc.identifier.bibliographicCitation | SENSORS, v.18, no.3 | - |
dc.relation.isPartOf | SENSORS | - |
dc.citation.title | SENSORS | - |
dc.citation.volume | 18 | - |
dc.citation.number | 3 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Engineering, Electrical & Electronic | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | EXPRESSED PROTEIN LIGATION | - |
dc.subject.keywordPlus | RESONANCE ENERGY-TRANSFER | - |
dc.subject.keywordPlus | GOLD NANOPARTICLE | - |
dc.subject.keywordPlus | SEMISYNTHESIS | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordPlus | COORDINATION | - |
dc.subject.keywordPlus | NANOSENSORS | - |
dc.subject.keywordPlus | FLUORESCENT | - |
dc.subject.keywordAuthor | luciferase | - |
dc.subject.keywordAuthor | bioluminescence | - |
dc.subject.keywordAuthor | matrix metalloproteinase | - |
dc.subject.keywordAuthor | intein | - |
dc.subject.keywordAuthor | biotinylation | - |
dc.identifier.url | https://www.mdpi.com/1424-8220/18/3/875 | - |
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