In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells and In Vivo Bone Formation in Composite Nanofiber Meshes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ko, Eun Kyoung | - |
dc.contributor.author | Jeong, Sung In | - |
dc.contributor.author | Rim, Nae Gyune | - |
dc.contributor.author | Lee, Young Moo | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.contributor.author | Lee, Bu-Kyu | - |
dc.date.accessioned | 2022-12-21T00:07:01Z | - |
dc.date.available | 2022-12-21T00:07:01Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-12 | - |
dc.identifier.issn | 1937-3341 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177582 | - |
dc.description.abstract | Tissue engineering has become an alternative method to traditional surgical treatments for the repair of bone defects, and an appropriate scaffold supporting bone formation is a key element in this approach. In the present study, nanofibrous organic and inorganic composite scaffolds containing nano-sized demineralized bone powders (DBPs) with biodegradable poly(L-lactide) (PLA) were developed using an electrospinning process for engineering bone. To assess their biocompatibility, in vitro osteogenic differentiation of human mandible-derived mesenchymal stem cells (hMSCs) cultured on PLA or PLA/DBP composite nanofiber scaffolds were examined. The mineralization of hMSCs cultured with osteogenic supplements on the PLA/DBP nanofiber scaffolds was remarkably greater than on the PLA nanofiber scaffold during the first 14 days of culture but reached the same level after 21 days. The in vivo osteoconductive effect of PLA/DBP nanofibrous scaffolds was further investigated using rats with critical-sized skull defects. Micro-computerized tomography revealed that a greater amount of newly formed bone extended across the defect area in PLA/DBP scaffolds than in the nonimplant and PLA scaffolds 12 weeks after implantation and that the defect size was almost 90% smaller. Therefore, PLA/DBP composite nanofiber scaffolds may serve as a favorable matrix for the regeneration of bone tissue. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells and In Vivo Bone Formation in Composite Nanofiber Meshes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Young Moo | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1089/ten.tea.2008.0057 | - |
dc.identifier.scopusid | 2-s2.0-56549121685 | - |
dc.identifier.wosid | 000261403500017 | - |
dc.identifier.bibliographicCitation | TISSUE ENGINEERING PART A, v.14, no.12, pp.2105 - 2119 | - |
dc.relation.isPartOf | TISSUE ENGINEERING PART A | - |
dc.citation.title | TISSUE ENGINEERING PART A | - |
dc.citation.volume | 14 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2105 | - |
dc.citation.endPage | 2119 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | ALLOGRAFT | - |
dc.subject.keywordPlus | BIOCOMPATIBILITY | - |
dc.subject.keywordPlus | MINERALIZATION | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | MEMBRANES | - |
dc.subject.keywordPlus | PROMOTES | - |
dc.subject.keywordPlus | DEFECTS | - |
dc.identifier.url | https://www.liebertpub.com/doi/10.1089/ten.tea.2008.0057 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.