Improvement of Differentiation and Mineralization of Pre-Osteoblasts on Composite Nanofibers of Poly(lactic acid) and Nanosized Bovine Bone Powder

Abstract

The effect of NBM incorporation in PLA nanofibers on their mechanical properties and the differentiation and mineralization of osteoblasts was studied. At 20% NBM, the Young's modulus of the nanofibers was 37.78 +/- 4.23, significantly larger than that of pure PLA nanofibers. MCM-E1 pre-osteoblasts attached to both types of nanofibers and developed full osteogenic phenotypes. A profound effect of NBM on the mineralization of MCM-E1 pre-osteoblasts was confirmed, suggesting that NBM/PLA composite nanofibers exhibit properties similar to those of the native collagen-rich mineralized bone matrix, and could therefore serve as a temporary substrate for facilitating the differentiation and mineralization of bone-forming cells.