Effect of Mobile Phase Composition on Henry's Constants of 2-Amino-3-phenyl-propanoic Acid, 2-Amino-3-(3-indolyl)-propanoic Acid, and 2-Amino-3-(4-hydroxyphenyl)-propanoic Acid in a Capcell Pak C-18 Chromatography
- Authors
- Han, Qiao; Yoo, Chang Geun; Jo, Se-Hee; Yi, Sung Chul; Mun, Sungyong
- Issue Date
- Nov-2008
- Publisher
- AMER CHEMICAL SOC
- Citation
- JOURNAL OF CHEMICAL AND ENGINEERING DATA, v.53, no.11, pp.2613 - 2621
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CHEMICAL AND ENGINEERING DATA
- Volume
- 53
- Number
- 11
- Start Page
- 2613
- End Page
- 2621
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177730
- DOI
- 10.1021/je800510y
- ISSN
- 0021-9568
- Abstract
- The adsorption equilibria necessary for the design of a chromatographic process for amino acids separation were measured on a Capcell Pak C-18 resin using a dynamic frontal analysis method. The single-component adsorption data of 2-amino-3-phenyl-propanoic acid, 2-amino-3-(3-indolyl)-propanoic acid, and 2-amino-3-(4-hydroxyphenyl)-propanoic acid were obtained at room temperature while varying the methanol content in the mobile phase from 5 % (v/v) to 70 % (v/v). On the basis of the resultant adsorption data, the Henry's constant, which is the ratio of solid-phase and liquid-phase concentrations at equilibrium, was determined for each amino acid and each mobile-phase composition. There was a large difference in Henry's constant among the three amino acids investigated. In addition, the Henry's constant of each amino acid showed a significant dependence on the mobile-phase composition. To correlate such a behavior, several models were tested. It was found that Abel's model containing three parameters was the most successful in correlating the Henry's constant of each amino acid as a function of mobile-phase composition. The data and model parameters reported in this study can be of use to the design of a chromatographic separation process based on a solvent gradient operation mode.
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