Di-(2-ethylhexyl) phthalate enhances melanoma tumor growth via differential effect on M1-and M2-polarized macrophages in mouse model
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae-Wook | - |
dc.contributor.author | Park, Sojin | - |
dc.contributor.author | Han, Hae-Kyoung | - |
dc.contributor.author | Gye, Myung Chan | - |
dc.contributor.author | Moon, Eun-Yi | - |
dc.date.accessioned | 2021-08-02T13:53:13Z | - |
dc.date.available | 2021-08-02T13:53:13Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-02 | - |
dc.identifier.issn | 0269-7491 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/17808 | - |
dc.description.abstract | Phthalates are widely used as plasticizers that influence sexual and reproductive development. Here, we investigated whether di-(2-ethylhexyl) phthalate (DEHP) affects macrophage polarization that are associated with tumor initiation and progression. No changes were observed in LPS- or ConA-stimulated in vitro spleen B or T cell proliferation for 48 h, respectively. In contrast, macrophage functions were inhibited in response to DEHP for 12 h as judged by LPS-induced H2O2 and NO production and zymosan A-mediated phagocytosis. When six weeks old male mice were pre-exposed to 4.0 mg/kg DEHP for 21 days before the injection of B16F10 melanoma cells and post-exposed to 4.0 mg/kg DEHP for 7 days, tumor nodule formation and the changes in tumor volume were higher than those in control group. Furthermore, when male mice were intraperitoneally pretreated with DEHP for 3 or 4 weeks and peritoneal exudate cells (PECs) or bone marrow-derived macrophages (BMDMs) were incubated with lipopolysaccharide (LPS), the expression of COX-2, TNF-alpha, and IL-6 was reduced in DEHP-pretreated cells as compared with that in LPS-stimulated control cells. While the production of nitric oxide (NO) for 18 h was reduced by LPS-stimulated PECs and M1-type BMDMs, IL-4 expression was enhanced in LPS-stimulated BMDMs. When BMDMs were incubated with IL-4 for 30 h, arginase 1 for M2-type macrophages was increased in transcriptional and translational level. Data implicate that macrophages were differentially polarized by DEHP treatment, which reduced M1-polarzation but enhanced M2-polarization. Taken together, these data demonstrate that DEHP could affect in vivo immune responses of macrophages, leading to the suppression of their tumor-preventing ability. This suggests that individuals at high risk for tumor incidence should avoid long-term exposure to various kind of phthalate including DEHP. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Di-(2-ethylhexyl) phthalate enhances melanoma tumor growth via differential effect on M1-and M2-polarized macrophages in mouse model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gye, Myung Chan | - |
dc.identifier.doi | 10.1016/j.envpol.2017.10.030 | - |
dc.identifier.scopusid | 2-s2.0-85033561684 | - |
dc.identifier.wosid | 000424177000088 | - |
dc.identifier.bibliographicCitation | ENVIRONMENTAL POLLUTION, v.233, pp.833 - 843 | - |
dc.relation.isPartOf | ENVIRONMENTAL POLLUTION | - |
dc.citation.title | ENVIRONMENTAL POLLUTION | - |
dc.citation.volume | 233 | - |
dc.citation.startPage | 833 | - |
dc.citation.endPage | 843 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.subject.keywordPlus | NITRIC-OXIDE PRODUCTION | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | IMMUNOSUPPRESSIVE ACTIVITY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | NONYLPHENOL | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DEHP | - |
dc.subject.keywordPlus | 4-NONYLPHENOL | - |
dc.subject.keywordAuthor | Di-(2-ethylhexyl) phthalate (DEHP) | - |
dc.subject.keywordAuthor | Macrophage polarization | - |
dc.subject.keywordAuthor | M1-type | - |
dc.subject.keywordAuthor | M2-type | - |
dc.subject.keywordAuthor | Endocrine disruptor | - |
dc.subject.keywordAuthor | Tumor growth | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0269749117321255?via%3Dihub | - |
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