Characteristics of detrusor overactivity induced by intravesical instillation of adenosine 5′-triphosphate
- Authors
- Kim, Sang Jin; Mon, Hong Sang; Choi, Hong Yong; Kim, Yong Tae
- Issue Date
- May-2008
- Publisher
- Korean Journal of Urology
- Keywords
- Adenosine triphosphate; Bladder; Detrusor overactivity; Rats
- Citation
- Korean Journal of Urology, v.49, no.5, pp.424 - 431
- Indexed
- SCOPUS
- Journal Title
- Korean Journal of Urology
- Volume
- 49
- Number
- 5
- Start Page
- 424
- End Page
- 431
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178639
- DOI
- 10.4111/kju.2008.49.5.424
- ISSN
- 0494-4747
- Abstract
- Purpose: The bladder urothelium releases adenosine 5′-triphosphate (ATP) with mechanical stretch, which increases in a pathologic state such as a neurogenic bladder. We investigated the characteristics of detrusor overactivity induced by the intravesical instillation of ATP. Materials and Methods: Under urethane anesthesia, cystometry was performed on female Sprague-Dawley rats. After pretreatment with protamine sulfate (PS, 10mg/ml), ATP (60mM, pH 6.0) was continuously infused intravesically. The effects of intravenous injection of pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid (PPADS) on ATP-induced detrusor overactivity were studied. Changes in cystometric parameters including parameters of each voiding phase, including bladder pressure increase rate (slope; Ph1slope), maximal bladder pressure (Ph1MBP, Ph2MBP, Ph3MBP), mean bladder pressure (Ph2meanBP) and variation of intraluminal pressure high-frequency oscillations (IPHFOs) were analysed. Results: With the intravesical instillation of ATP after PS pretreatment, the intercontraction interval (ICI) decreased significantly (544.2±167.2s vs 222.3±170.8s, p=0.001), maximal voiding pressure (MVP) decreased significantly (31.3±4.7cmH2O vs 25±2.9cmH2O, p=0.001), and pressure threshold (PT) decreased significantly (4.0±1.3cmH2O vs 1.4±0.8cmH2O, P=0.001). Changes in the ICI, MVP & PT induced by intravesical instillation of ATP were reversed by intravenous injection of PPADS. Ph1slope, Ph1MBP, Ph2MBP, Ph2meanBP and Ph3MBP were decreased, but IPHFOs were not affected by intravesical application of ATP. Conclusions: Intravesical instillation of ATP in female rats induced detrusor overactivity, which may be mediated by acting on the urine storage phase.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 비뇨의학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178639)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.