Inhibition of glycogen synthase kinase-3 reduces L-DOPA-induced neurotoxicity
DC Field | Value | Language |
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dc.contributor.author | Koh, Seong-Ho | - |
dc.contributor.author | Song, Chiwon | - |
dc.contributor.author | Noh, Min Young | - |
dc.contributor.author | Kim, Hyun Young | - |
dc.contributor.author | Lee, Kyu-Yong | - |
dc.contributor.author | Lee, Young Joo | - |
dc.contributor.author | Kim, Juhan | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.contributor.author | Kim, Hee-Tae | - |
dc.date.accessioned | 2022-12-21T03:26:00Z | - |
dc.date.available | 2022-12-21T03:26:00Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-05 | - |
dc.identifier.issn | 0300-483X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/178705 | - |
dc.description.abstract | The neurotoxicity of L-3,4-dihydroxyphenylalanine (L-DOPA), used for the treatment of Parkinson's disease, remains controversial. Although there are many reports suggesting that long-term treatment of L-DOPA causes neuronal death, an increasing body of recent evidence has proposed that L-DOPA might be neuroprotective rather than neurotoxic. We investigated the effect Of L-DOPA on neuronally differentiated PC12 (nPC12) cells by treating cells with various concentrations Of L-DOPA for 24h. We also studied whether glycogen synthase kinase (GSK)-3 activation is related to L-DOPA-induced neurotoxicity by simultaneously treating cells with several concentrations Of L-DOPA and a GSK-3 inhibitor for 24 h. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, trypan blue staining, cell counting kit-8, and DAPI staining all showed that L-DOPA decreased nPC12 cell viability at high concentrations. In addition, 100 mu M L-DOPA treatment significantly increased the activity of GSK-3 and death signals including cytochrome c, activated caspase-3 and cleaved PARP, and decreased survival signals including heat shock transcription factor-1 in a concentration-dependent manner. Treatment with GSK-3 inhibitor VIII or lithium chloride prevented L-DOPA-induced cell death. Together, these results suggest that L-DOPA induces neuronal cell death at high concentrations and that the neurotoxic effect Of L-DOPA might be mediated in part by GSK-3 activation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.title | Inhibition of glycogen synthase kinase-3 reduces L-DOPA-induced neurotoxicity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Koh, Seong-Ho | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Young | - |
dc.contributor.affiliatedAuthor | Lee, Kyu-Yong | - |
dc.contributor.affiliatedAuthor | Lee, Young Joo | - |
dc.contributor.affiliatedAuthor | Kim, Seung Hyun | - |
dc.contributor.affiliatedAuthor | Kim, Hee-Tae | - |
dc.identifier.doi | 10.1016/j.tox.2008.02.007 | - |
dc.identifier.scopusid | 2-s2.0-42749091984 | - |
dc.identifier.wosid | 000256208400006 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY, v.247, no.2-3, pp.112 - 118 | - |
dc.relation.isPartOf | TOXICOLOGY | - |
dc.citation.title | TOXICOLOGY | - |
dc.citation.volume | 247 | - |
dc.citation.number | 2-3 | - |
dc.citation.startPage | 112 | - |
dc.citation.endPage | 118 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | PARKINSONS-DISEASE | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | PHOSPHATIDYLINOSITOL 3-KINASE | - |
dc.subject.keywordPlus | NEURONAL DEATH | - |
dc.subject.keywordPlus | LEVODOPA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | GLYCOGEN-SYNTHASE-KINASE-3 | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordAuthor | L-DOPA | - |
dc.subject.keywordAuthor | glycogen synthase kinase-3 | - |
dc.subject.keywordAuthor | neurotoxicity | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0300483X08000838?via%3Dihub | - |
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