Neurochemical characterization of the TRPV1-Positive nociceptive, primary Afferents innervating skeletal muscles in the rats
DC Field | Value | Language |
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dc.contributor.author | Shin, Dong Su | - |
dc.contributor.author | Kim, Eun Hyun | - |
dc.contributor.author | Song, Kwan Young | - |
dc.contributor.author | Hong, Hyun Jong | - |
dc.contributor.author | Kong, Min Ho | - |
dc.contributor.author | Hwang, Se Jin | - |
dc.date.accessioned | 2022-12-21T04:20:34Z | - |
dc.date.available | 2022-12-21T04:20:34Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-02 | - |
dc.identifier.issn | 2005-3711 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179004 | - |
dc.description.abstract | Objective : Transient receptor potential vanilloid subfamily type 1 (TRPV1), a most specific marker of the nociceptive primary afferent, is expressed in peptidergic and non-pepetidergic primary afferents innervating skin and viscera. However, its expression in sensory fibers to skeletal muscle is not well known. In this study, we studied the neurochemical characteristics of TRPV1-positive primary afferents to skeletal muscles. Methods : Sprague-Dawley rats were injected with total 20 mu l of 1% fast blue (FB) into the gastrocnemius and erector spinae muscle and animals were perfused 4 days after injection. FB-positive cells were traced in the L4-L5 (for gastrocnemius muscle) and L2-L4 (for erector spinae muscle) dorsal root ganglia. The neuro-chemical characteristics of the muscle afferents were studied with multiple immunofluorescence with TRPV1, calcitonin gene-related peptide ( CGRP) and P2X(3). To identify spinal neurons responding to noxious stimulus to the skeletal muscle, 10% acetic acids were injected into the gastrocnemius and erector spinae muscles and expression of phospho extracellular signal-regulated kinase (pERK) in spinal cords were identified with immunohistochemical method. Results: TRPV1 was expressed in about 49% of muscle afferents traced from gastrocnemius and 40% of erector spinae. Sixty-five to 60% of TRPV1-positive muscles afferents also expressed CGRP. In contrast, expression of P2X(3) immnoreaction in TRPV1-positive muscle afferents were about 20%. TRPV1-positive primary afferents were contacted with spinal neurons expressing pERK after injection of acetic acid into the muscles. Conclusion : It is consequently suggested that nociception from skeletal muscles are mediated by TRPV1-positive primary afferents and majority of them are also peptidergic. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN NEUROSURGICAL SOC | - |
dc.title | Neurochemical characterization of the TRPV1-Positive nociceptive, primary Afferents innervating skeletal muscles in the rats | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Se Jin | - |
dc.identifier.doi | 10.3340/jkns.2008.43.2.97 | - |
dc.identifier.wosid | 000254582500005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY, v.43, no.2, pp.97 - 104 | - |
dc.relation.isPartOf | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY | - |
dc.citation.title | JOURNAL OF KOREAN NEUROSURGICAL SOCIETY | - |
dc.citation.volume | 43 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 97 | - |
dc.citation.endPage | 104 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001248089 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Clinical Neurology | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | ROOT GANGLION NEURONS | - |
dc.subject.keywordPlus | ACTIVATED PROTEIN-KINASE | - |
dc.subject.keywordPlus | LUMBOSACRAL SPINAL-CORD | - |
dc.subject.keywordPlus | SUBSTANCE-P | - |
dc.subject.keywordPlus | DORSAL-ROOT | - |
dc.subject.keywordPlus | CAPSAICIN RECEPTOR | - |
dc.subject.keywordPlus | GASTROCNEMIUS-MUSCLE | - |
dc.subject.keywordPlus | NOXIOUS-STIMULATION | - |
dc.subject.keywordPlus | NEUROPATHIC PAIN | - |
dc.subject.keywordPlus | URINARY-TRACT | - |
dc.subject.keywordAuthor | skeletal muscle | - |
dc.subject.keywordAuthor | pain | - |
dc.subject.keywordAuthor | primary afferents | - |
dc.subject.keywordAuthor | TRPV1 | - |
dc.subject.keywordAuthor | CGRP | - |
dc.subject.keywordAuthor | dorsal root ganglion. | - |
dc.identifier.url | https://www.jkns.or.kr/journal/view.php?doi=10.3340/jkns.2008.43.2.97 | - |
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