Augmentation of erythropoietin enhancer-mediated hypoxia-inducible gene expression by co-transfection of a plasmid encoding hypoxia-inducible factor 1 for ischemic tissue targeting gene therapy
DC Field | Value | Language |
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dc.contributor.author | Lee, Suyeon | - |
dc.contributor.author | Kim, Kyunghwa | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-12-21T04:51:02Z | - |
dc.date.available | 2022-12-21T04:51:02Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2008-01 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179099 | - |
dc.description.abstract | Therapeutic angiogenesis with gene encoding vascular endothelial growth factor (VEGF) is a potential treatment for ischemic diseases. However, VEGF expression should be tightly regulated to avoid side effects such as tumor growth. Previously, our group developed the erythropoietin (Epo) enhancer-SV40 promoter system for hypoxia-specific gene expression. In the present study, the activity of the Epo enhancer-SV40 promoter system was further enhanced without significant decrease in its specificity by co-transfection of the hypoxia-inducible factor 1 alpha (HIF1 alpha) gene. pSV-HIF1 alpha was constructed by the insertion of the HIF1 alpha cDNA into pSI. At a 1:1 ratio, co-transfection of pSV-HIF1 alpha and pEpo-SV-Luc increased the promoter activity of the Epo enhancer-SV40 promoter system, showing at least three times higher gene expression under hypoxia as compared with the pEpo-SV-Luc single-plasmid transfection. Furthermore, co-transfection showed significant hypoxia specificity. Also, co-transfection of pEpo-SV-VEGF with pSV-HIF1 alpha showed the enhanced VEGF expression without loss of hypoxia specificity, as compared with pEpo-SV-VEGF single-plasmid transfection. Furthermore, pSV-HIF1 alpha induced the endogenous hypoxia-responsive genes such as angiopoietin-1, which would be beneficial for therapeutic angiogenesis. Therefore, with hypoxia specificity and higher gene expression, co-transfection of pSV-HIF1 alpha and pEpo-SV-VEGF may be useful for ischemia targeting gene therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Augmentation of erythropoietin enhancer-mediated hypoxia-inducible gene expression by co-transfection of a plasmid encoding hypoxia-inducible factor 1 for ischemic tissue targeting gene therapy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1080/10611860701699693 | - |
dc.identifier.scopusid | 2-s2.0-38049075353 | - |
dc.identifier.wosid | 000252351200005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.16, no.1, pp.43 - 50 | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 16 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 43 | - |
dc.citation.endPage | 50 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | ENDOTHELIAL GROWTH-FACTOR | - |
dc.subject.keywordPlus | CHRONIC MYOCARDIAL-ISCHEMIA | - |
dc.subject.keywordPlus | WATER-SOLUBLE LIPOPOLYMER | - |
dc.subject.keywordPlus | MESSENGER-RNA STABILITY | - |
dc.subject.keywordPlus | HIF-1-ALPHA/VP16 HYBRID | - |
dc.subject.keywordPlus | SKELETAL-MUSCLE | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | VEGF | - |
dc.subject.keywordAuthor | angiogenic growth factor | - |
dc.subject.keywordAuthor | erythropoietin enhancer | - |
dc.subject.keywordAuthor | hypoxia-inducible factor 1 alpha | - |
dc.subject.keywordAuthor | ischemia | - |
dc.subject.keywordAuthor | transcription regulation | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/10611860701699693 | - |
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