Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun Jeong | - |
dc.contributor.author | Bae, In Young | - |
dc.contributor.author | Ahn, Chang-Won | - |
dc.contributor.author | Lee, Suyong | - |
dc.contributor.author | Lee, Hyeon Gyu | - |
dc.date.accessioned | 2022-12-21T05:27:50Z | - |
dc.date.available | 2022-12-21T05:27:50Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-11 | - |
dc.identifier.issn | 0196-9781 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179369 | - |
dc.description.abstract | Adipogenesis inhibitory peptide was isolated and identified from black soybean (Rhynchosia volubilis Lour.) hydrolysate. An adipogenesis inhibitor was purified using consecutive methods including: ultrafiltration (MWCO; 3 and 10 kDa), gel filtration chromatography (Superdex Peptide 10/300 GL column), and reverse-phase high-performance liquid chromatography (mu Bondapak(TM) C-18 column). Also, the adipogenesis inhibition effect of the purified peptide was measured by observation of droplet of 3T3-L1 adipocyte by Oil Red O staining in the highest active fraction in each step. The peptide was shown to inhibit the differentiation of the 3T3-L1 pre-adipocyte, which was confirmed by morphological study. The adipogenesis inhibitory peptide was purified 71.43-fold from black soybean hydrolysate throughout a five-step purification procedure. The adipogenesis inhibitor was identified to be a tripeptide, Ile-Gln-Asn, having an IC50 value of 0.014 mg protein/ml. Furthermore, the synthetic tripeptide (Ile-Gln-Asn) exhibited the similar adipogenesis effects to the purified peptide. Thus, these results showed the potential anti-obesity effect of the purified peptide through control of adiposity. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.title | Purification and identification of adipogenesis inhibitory peptide from black soybean protein hydrolysate | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Hyeon Gyu | - |
dc.identifier.doi | 10.1016/j.peptides.2007.08.030 | - |
dc.identifier.scopusid | 2-s2.0-35348991559 | - |
dc.identifier.wosid | 000251104300002 | - |
dc.identifier.bibliographicCitation | PEPTIDES, v.28, no.11, pp.2098 - 2103 | - |
dc.relation.isPartOf | PEPTIDES | - |
dc.citation.title | PEPTIDES | - |
dc.citation.volume | 28 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 2098 | - |
dc.citation.endPage | 2103 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | WEIGHT-REDUCTION | - |
dc.subject.keywordPlus | SOY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordAuthor | black soybean peptide | - |
dc.subject.keywordAuthor | adipogenesis inhibitor | - |
dc.subject.keywordAuthor | 3T3-L1 cell | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0196978107003567?via%3Dihub | - |
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