IL-1Ra elaboration by colchicine stimulation in normal human bronchial epithelial cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae Hyung | - |
dc.contributor.author | Kim, Sang Heon | - |
dc.contributor.author | Kim, Tae Hyung | - |
dc.contributor.author | Sohn, Jang Won | - |
dc.contributor.author | Yoon, Ho Joo | - |
dc.contributor.author | Shin, Dong Ho | - |
dc.contributor.author | Park, Sung Soo | - |
dc.date.accessioned | 2022-12-21T06:55:18Z | - |
dc.date.available | 2022-12-21T06:55:18Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2007-08 | - |
dc.identifier.issn | 1738-3536 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179752 | - |
dc.description.abstract | Background: Asthma is a syndrome that is characterized by a variable degree of airflow obstruction, bronchial hyperresponsiveness, and airway inflammation Colchicine is an inexpensive and safe medication with unique anti-inflammatory properties. IL-1Ra (Interleukin-1 receptor antagonist) mediates the anti-inflammatory effect in human inflammatory diseases, including asthma. This study examined whether IL-1Ra mediates the anti-inflammatory effect of colchicine in normal human bronchial epithelial cells (NHBE), RAW 264.7 cells (murine macrophage cell line), and a mouse lung. Methods: NHBE, RAW 264.7 cells and BALB/c mice were stimulated with colchicine, and the increase in the IL-1Ra level was estimated by ELISA, Western analysis and RT-PCR analysis. Results: Colchicine stimulated NHBE and RAW 264.7 cells to release IL-1Ra into the supernatant in a dose-and time-dependent manner. The major isoform of IL-1Ra in NHBE and RAW 264.7 cells is type I icIL-1Ra, and sIL-1Ra, respectively. IL-1Ra up-regulation was blocked by PD98059, a specific inhibitor in MAPK pathways. Colchicine also stimulated the secretion of IL-1Ra into the bronchoalveolar lavage (BAL) fluid of BALB/c mouse. Conclusion: Colchicine stimulates an increase in the IL-1Ra level both in vivo and in vitro, and might have an anti-inflammatory effect. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | Korean National Tuberculosis Association | - |
dc.title | IL-1Ra elaboration by colchicine stimulation in normal human bronchial epithelial cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Tae Hyung | - |
dc.contributor.affiliatedAuthor | Sohn, Jang Won | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Joo | - |
dc.identifier.doi | 10.4046/trd.2007.63.2.145 | - |
dc.identifier.scopusid | 2-s2.0-36549019130 | - |
dc.identifier.bibliographicCitation | Tuberculosis and Respiratory Diseases, v.63, no.2, pp.145 - 153 | - |
dc.relation.isPartOf | Tuberculosis and Respiratory Diseases | - |
dc.citation.title | Tuberculosis and Respiratory Diseases | - |
dc.citation.volume | 63 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 145 | - |
dc.citation.endPage | 153 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001075641 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.subject.keywordPlus | 2 (2 amino 3 methoxyphenyl)chromone | - |
dc.subject.keywordPlus | colchicine | - |
dc.subject.keywordPlus | interleukin 1 receptor blocking agent | - |
dc.subject.keywordPlus | mitogen activated protein kinase inhibitor | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | antiinflammatory activity | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | bronchus mucosa | - |
dc.subject.keywordPlus | cell line | - |
dc.subject.keywordPlus | concentration response | - |
dc.subject.keywordPlus | cytokine release | - |
dc.subject.keywordPlus | enzyme linked immunosorbent assay | - |
dc.subject.keywordPlus | epithelium cell | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | lung lavage | - |
dc.subject.keywordPlus | macrophage | - |
dc.subject.keywordPlus | mouse | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | reverse transcription polymerase chain reaction | - |
dc.subject.keywordPlus | upregulation | - |
dc.subject.keywordPlus | Western blotting | - |
dc.subject.keywordAuthor | Asthma | - |
dc.subject.keywordAuthor | Colchicine | - |
dc.subject.keywordAuthor | IL-1Ra | - |
dc.subject.keywordAuthor | MAPK | - |
dc.identifier.url | https://www.e-trd.org/journal/view.php?doi=10.4046/trd.2007.63.2.145 | - |
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