Transvascular delivery of small interfering RNA to the central nervous system
DC Field | Value | Language |
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dc.contributor.author | Kumar, Priti | - |
dc.contributor.author | Wu, Haoquan | - |
dc.contributor.author | McBride, Jodi L. | - |
dc.contributor.author | Jung, Kyeong-Eun | - |
dc.contributor.author | Kim, Moon Hee | - |
dc.contributor.author | Davidson, Beverly L. | - |
dc.contributor.author | Lee, Sang Kyung | - |
dc.contributor.author | Shankar, Premlata | - |
dc.contributor.author | Manjunath, N. | - |
dc.date.accessioned | 2022-12-21T07:19:20Z | - |
dc.date.available | 2022-12-21T07:19:20Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-07 | - |
dc.identifier.issn | 0028-0836 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/179898 | - |
dc.description.abstract | A major impediment in the treatment of neurological diseases is the presence of the blood - brain barrier, which precludes the entry of therapeutic molecules from blood to brain. Here we show that a short peptide derived from rabies virus glycoprotein (RVG) enables the transvascular delivery of small interfering RNA ( siRNA) to the brain. This 29-amino-acid peptide specifically binds to the acetylcholine receptor expressed by neuronal cells. To enable siRNA binding, a chimaeric peptide was synthesized by adding nonamer arginine residues at the carboxy terminus of RVG. This RVG-9R peptide was able to bind and transduce siRNA to neuronal cells in vitro, resulting in efficient gene silencing. After intravenous injection into mice, RVG-9R delivered siRNA to the neuronal cells, resulting in specific gene silencing within the brain. Furthermore, intravenous treatment with RVG-9R-bound antiviral siRNA afforded robust protection against fatal viral encephalitis in mice. Repeated administration of RVG-9R-bound siRNA did not induce inflammatory cytokines or anti-peptide antibodies. Thus, RVG-9R provides a safe and noninvasive approach for the delivery of siRNA and potentially other therapeutic molecules across the blood - brain barrier. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Transvascular delivery of small interfering RNA to the central nervous system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang Kyung | - |
dc.identifier.doi | 10.1038/nature05901 | - |
dc.identifier.scopusid | 2-s2.0-34447114618 | - |
dc.identifier.wosid | 000247720900032 | - |
dc.identifier.bibliographicCitation | NATURE, v.448, no.7149, pp.39 - 43 | - |
dc.relation.isPartOf | NATURE | - |
dc.citation.title | NATURE | - |
dc.citation.volume | 448 | - |
dc.citation.number | 7149 | - |
dc.citation.startPage | 39 | - |
dc.citation.endPage | 43 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | CELL-PENETRATING PEPTIDES | - |
dc.subject.keywordPlus | INHIBITS TUMOR-GROWTH | - |
dc.subject.keywordPlus | IN-VIVO DELIVERY | - |
dc.subject.keywordPlus | INTRACELLULAR DELIVERY | - |
dc.subject.keywordPlus | MODIFIED SIRNAS | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | VIRUS | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | TRANSPORTERS | - |
dc.identifier.url | https://www.nature.com/articles/nature05901 | - |
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