Generation of functional dopamine neurons from neural precursor cells isolated from the subventricular zone and white matter of the adult rat brain using Nurr1 overexpression
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shim, Jae Won | - |
dc.contributor.author | Park, Chang Hwan | - |
dc.contributor.author | Bae, Yong Chul | - |
dc.contributor.author | Bae, Jin Young | - |
dc.contributor.author | Chung, Seungsoo | - |
dc.contributor.author | Chang, Mi Yoon | - |
dc.contributor.author | Koh, Hyun Chul | - |
dc.contributor.author | Lee, Hyun Seob | - |
dc.contributor.author | Hwang, Se Jin | - |
dc.contributor.author | Lee, Ki Hwan | - |
dc.contributor.author | Lee, Yong-Sung | - |
dc.contributor.author | Choi, Cha-Yong | - |
dc.contributor.author | Lee, Sang-Hun | - |
dc.date.accessioned | 2022-12-21T08:16:40Z | - |
dc.date.available | 2022-12-21T08:16:40Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.issn | 1066-5099 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180142 | - |
dc.description.abstract | Neural precursor (NP) cells from adult mammalian brains can be isolated, expanded in vitro, and potentially used as cell replacement source material for treatment of intractable brain disorders. Reduced ethical concerns, lack of teratoma formation, and possible ex vivo autologous transplantation are critical advantages to using adult NP donor cells over cells from fetal brain tissue or embryonic stem cells. However, the usage of adult NP cells is limited by the ability to induce specific neurochemical phenotypes in these cells. Here, we demonstrate induction of a dopaminergic phenotype in NP cells isolated from the subventricular zone (SVZ) and white matter of rodent adult brains using overexpression of the nuclear receptor Nurr1 in vitro. Forced expression of Nurr1, a transcriptional factor specific to midbrain dopamine (DA) neuron development, caused in the adult cells an acquisition of the DA neurotransmitter phenotype and sufficient differentiation toward morphologically, phenotypically, and ultrastructurally mature DA neurons. Coexpression of neurogenic factor Mash1 and treatment with neurogenic cytokines brain-derived neurotrophic factor and neurotrophin-3 greatly enhanced Nurr1-induced DA neuron yield. The Nurr1-induced DA neurons demonstrated in vitro presynaptic DA neuronal functionality, releasing DA neurotransmitter in response to depolarization stimuli and specific DA reuptake. Furthermore, Nurr1-engineered adult SVZ NP cells survived, integrated, and differentiated into DA neurons in vivo that can reverse the behavioral deficit in the host striatum of parkinsonian rats. These findings open the possibility for the use of precursor cells from adult brains as a cell source for neuronal replacement treatment of Parkinson disease. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ALPHAMED PRESS | - |
dc.title | Generation of functional dopamine neurons from neural precursor cells isolated from the subventricular zone and white matter of the adult rat brain using Nurr1 overexpression | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Chang Hwan | - |
dc.contributor.affiliatedAuthor | Chang, Mi Yoon | - |
dc.contributor.affiliatedAuthor | Koh, Hyun Chul | - |
dc.contributor.affiliatedAuthor | Hwang, Se Jin | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Hun | - |
dc.identifier.doi | 10.1634/stemcells.2006-0274 | - |
dc.identifier.scopusid | 2-s2.0-34247629489 | - |
dc.identifier.wosid | 000246292700019 | - |
dc.identifier.bibliographicCitation | STEM CELLS, v.25, no.5, pp.1252 - 1262 | - |
dc.relation.isPartOf | STEM CELLS | - |
dc.citation.title | STEM CELLS | - |
dc.citation.volume | 25 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1252 | - |
dc.citation.endPage | 1262 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | LOOP-HELIX FACTORS | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | MESENCEPHALIC PRECURSORS | - |
dc.subject.keywordPlus | TYROSINE-HYDROXYLASE | - |
dc.subject.keywordPlus | PRONEURAL GENES | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | adult neural stem cell | - |
dc.subject.keywordAuthor | Nurr1 | - |
dc.subject.keywordAuthor | dopamine neurons | - |
dc.subject.keywordAuthor | Parkinson disease | - |
dc.identifier.url | https://academic.oup.com/stmcls/article/25/5/1252/6402264 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.