Phospholipase D1 activation through Src and Ras is involved in basic fibroblast growth factor-induced neurite outgrowth of H19-7 cells
DC Field | Value | Language |
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dc.contributor.author | Oh, Doo Yi | - |
dc.contributor.author | Park, Shin Young | - |
dc.contributor.author | Cho, Ju Hwan | - |
dc.contributor.author | Lee, Ki Sung | - |
dc.contributor.author | Min, Do Sik | - |
dc.contributor.author | Han, Joong Soo | - |
dc.date.accessioned | 2022-12-21T08:26:47Z | - |
dc.date.available | 2022-12-21T08:26:47Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-05 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180176 | - |
dc.description.abstract | Phospholipase D (PLD) is implicated in a variety of physiological processes that reveal it to be a member of the signal transducing phospholipases. We found that PLD1 is activated when basic fibroblast growth factor (bFGF) stimulates neurite outgrowth of an immortalized hippocampal cell line (H19-7). Overexpression of PLD1 in H19-7 cells dramatically elongated bFGF-induced neurite outgrowth and increased PLD activity. Transfection of DN-rPLD1 blocked bFGF-induced PLD activation and completely inhibited neurite outgrowth induced by bFGF, suggesting that PLD1 activation is important in bFGF-induced neurite outgrowth of H19-7 cells. PLD activation and neurite outgrowth induced by bFGF was dependent on phospholipase C gamma (PLC-gamma) and Ca2+, but not protein kinase C (PKC). Furthermore, inhibition of Src and Ras partially blocked bFGF-induced PLD activation and neurite outgrowth, respectively. Coinhibition of Src and Ras completely blocked bFGF-induced PLD activation, suggesting that Src and Ras independently regulate PLD1 activation. Interestingly, bFGF-induced PLD activation and neurite outgrowth did not require ERK1/2 activated by Ras. Taken together, this study demonstrates that bFGF activates PLD1 through PLC-gamma activation, which leads to neurite outgrowth in H19-7 cells. Furthermore, our results show that PLD1 activation by bFGF is regulated by Src and Ras independently. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-LISS | - |
dc.title | Phospholipase D1 activation through Src and Ras is involved in basic fibroblast growth factor-induced neurite outgrowth of H19-7 cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, Joong Soo | - |
dc.identifier.doi | 10.1002/jcb.21166 | - |
dc.identifier.scopusid | 2-s2.0-34247477686 | - |
dc.identifier.wosid | 000245909200019 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, v.101, no.1, pp.221 - 234 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.title | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.citation.volume | 101 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 221 | - |
dc.citation.endPage | 234 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | CENTRAL-NERVOUS-SYSTEM | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | PC12 CELLS | - |
dc.subject.keywordPlus | AUTOPHOSPHORYLATION SITES | - |
dc.subject.keywordPlus | TYROSINE PHOSPHORYLATION | - |
dc.subject.keywordPlus | NEURONAL DIFFERENTIATION | - |
dc.subject.keywordPlus | KINASE ACTIVATION | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | MAP KINASE | - |
dc.subject.keywordPlus | RECEPTOR 1 | - |
dc.subject.keywordAuthor | phospholipase D1 (PLD1) | - |
dc.subject.keywordAuthor | neurite outgrowth | - |
dc.subject.keywordAuthor | basic fibroblast growth factor (bFGF) | - |
dc.subject.keywordAuthor | Src | - |
dc.subject.keywordAuthor | Ras | - |
dc.subject.keywordAuthor | phospholipase | - |
dc.subject.keywordAuthor | C-gamma (PLC-gamma) | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/jcb.21166 | - |
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