Quantitative analysis of phosphinothricin-N-acetyltransferase in genetically modified herbicide tolerant pepper by an enzyme-linked immunosorbent assay
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shim, Youn Young | - |
dc.contributor.author | Shin, Weon Sun | - |
dc.contributor.author | Moon, Gi Seong | - |
dc.contributor.author | Kim, Kyung Hwan | - |
dc.date.accessioned | 2022-12-21T08:48:18Z | - |
dc.date.available | 2022-12-21T08:48:18Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180269 | - |
dc.description.abstract | An immunoassay method was developed to quantitatively detect phosphinothricin-N-acetyltransferase (PAT) encoded by the Bialaphos resistance (bar) gene in genetically modified (GM) pepper. The histidine-tagged PAT was overexpressed in Escherichia coli M15 (pQE31-bar) and efficiently purified by Ni2+ affinity chromatography. A developed sandwich enzyme-linked immunosorbent assay (S-ELISA) method (detection limit: 0.01 mu g/ml) was 100-fold more sensitive than a competitive indirect ELISA (CI-ELISA) method or Western blot analysis in detecting the recombinant PAT. In real sample tests, PAT in genetically modified herbicide-tolerant (GMHT) peppers was successfully quantified [4.9 +/- 0.4 mu g/g of sample (n=6)] by the S-ELISA method. The S-ELISA method developed here could be applied to other GMHT crops and vegetables producing PAT. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Quantitative analysis of phosphinothricin-N-acetyltransferase in genetically modified herbicide tolerant pepper by an enzyme-linked immunosorbent assay | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Weon Sun | - |
dc.identifier.scopusid | 2-s2.0-34248372107 | - |
dc.identifier.wosid | 000246096700021 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.4, pp.681 - 684 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 17 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 681 | - |
dc.citation.endPage | 684 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001051678 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | MODIFIED ORGANISMS | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | ELISA | - |
dc.subject.keywordPlus | FOOD | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordAuthor | phosphinothricin-N-acetyltransferase | - |
dc.subject.keywordAuthor | bar gene | - |
dc.subject.keywordAuthor | GM pepper | - |
dc.subject.keywordAuthor | sandwich ELISA | - |
dc.identifier.url | https://www.jmb.or.kr/journal/view.html?uid=2188&vmd=Full | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.