Non-ionic amphiphilic biodegradable PEG-PLGA-PEG copolymer enhances gene delivery efficiency in rat skeletal muscle
DC Field | Value | Language |
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dc.contributor.author | Chang, Chien Wen | - |
dc.contributor.author | Choi, Donghoon | - |
dc.contributor.author | Kim, Won Jong | - |
dc.contributor.author | Yockman, James W. | - |
dc.contributor.author | Christensen, Lane V. | - |
dc.contributor.author | Kim, Yong Hee | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.date.accessioned | 2022-12-21T08:49:57Z | - |
dc.date.available | 2022-12-21T08:49:57Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180284 | - |
dc.description.abstract | Naked plasmid DNA (pDNA)-based gene therapy has low delivery efficiency, and consequently, low therapeutic effect. We present a biodegradable nonionic triblock copolymer, PEG(13)-PLGA(10)-PEG(13), to enhance gene delivery efficiency in skeletal muscle. Effects of PEG(13)PLGA(10)-PEG(13) on physicochemical properties of pDNA were evaluated by atomic force microscopy (AFM) imaging, gel electrophoresis and zeta-potential analysis. AFM imaging suggested a slightly compacted structure of pDNA when it was mixed with the polymer, while zeta-potential measurement indicated an increased surface potential of negatively charged pDNA. PEG(13)-PLGA(10)-PEG(13) showed a relatively lower toxicity compared to Pluronic P85 in a skeletal muscle cell line. The luciferase expression of pDNA delivered in 0.25% polymer solution was up to three orders of magnitude more than branched polyethylenimine (bPEI(25 k))/pDNA and three times more than that of naked pDNA five days after intramuscular administration. This in vivo gene delivery enhancement was also observed displaying a two-fold higher expression of human vascular endothelial growth factor (VEGF). Based on fluorescence labeled pDNA distribution, it is speculated that the greater diffusivity of PEG(13)-PLGA(10)-PEG(13)/pDNA compared to bPET(25 k)/pDNA accounts for better transfection efficiency in vivo. To summarize, combining PEG(13)-PLGA(10)-PEG(13) with pDNA possesses the potential to improve gene delivery efficiency in skeletal muscle. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Non-ionic amphiphilic biodegradable PEG-PLGA-PEG copolymer enhances gene delivery efficiency in rat skeletal muscle | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Yong Hee | - |
dc.identifier.doi | 10.1016/j.jconrel.2006.11.025 | - |
dc.identifier.scopusid | 2-s2.0-33847353779 | - |
dc.identifier.wosid | 000245498500012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.118, no.2, pp.245 - 253 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 118 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 245 | - |
dc.citation.endPage | 253 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | WATER-SOLUBLE LIPOPOLYMER | - |
dc.subject.keywordPlus | GROWTH-FACTOR GENE | - |
dc.subject.keywordPlus | BLOCK-COPOLYMERS | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | THERAPEUTIC ANGIOGENESIS | - |
dc.subject.keywordPlus | INTRAMUSCULAR INJECTION | - |
dc.subject.keywordPlus | LIMB ISCHEMIA | - |
dc.subject.keywordPlus | MOUSE MUSCLE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | gene therapy | - |
dc.subject.keywordAuthor | PEG-PLGA-PEG | - |
dc.subject.keywordAuthor | skeletal muscle | - |
dc.subject.keywordAuthor | plasmid DNA | - |
dc.subject.keywordAuthor | VEGF | - |
dc.subject.keywordAuthor | biodegradable | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365906006791?via%3Dihub | - |
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