Surface-grafting of polyglutamate on Si wafer using micro contact printing

Abstract

Surface- grafting of poly(gamma-benzyl-alpha, L- glutamate) ( PBLG) on the 3- aminopropyl triethoxysilane ( APS) pretreated silicone substrates was conducted by the monomer, c- benzyl-L-glutamate N-carboxyanhydride ( BLG-NCA) which was synthesized through the phosgenation procedure. PBLG, one of liquid crystalline polymers, micro-patterns were fabricated by micro-contact printing (mu CP) and surfacegrafting method. Then, PBLG patterns were characterized with electron microscopy ( SEM), atomic force microscopy ( AFM), infrared spectroscopy (FT-IR), Raman spectroscopy and polarized optical microscopy. alpha-helix conformation of PBLG was dominated on the patterned surface which was confirmed by FT-IR spectroscopy. From the polarizing microscope images, lyotropic liquid crystalline properties of the patterned surfaces were observed. The SEM and AFM images show that the height of PBLG patterns was 300nm and line width was 15 mu m. The interval between lines was about 5 mu m.