DNA delivery to the mitochondria sites using mitochondrial leader peptide conjugated polyethylenimine
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Choi, Joon Sig | - |
dc.contributor.author | Choi, Min Ji | - |
dc.contributor.author | Pak, Youngmi Kim | - |
dc.contributor.author | Rhee, Byoung Doo | - |
dc.contributor.author | Ko, Kyung Soo | - |
dc.date.accessioned | 2022-12-21T09:09:41Z | - |
dc.date.available | 2022-12-21T09:09:41Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-02 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180472 | - |
dc.description.abstract | Some genetic diseases are associated with the defects of the mitochondrial genome. Direct DNA delivery to the mitochondrial matrix has been suggested as an approach for mitochondrial gene therapy for these diseases. We hypothesized that a mitochondrial leader peptide (LP) conjugated polyethylenimine (PEI) could deliver DNA to the mitochondrial sites. PEI-LP was synthesized by the conjugation of LP to PEI using disulfide bond. The complex formation of PEI-LP with DNA was confirmed by a gel retardation assay. In this study, DNA was completely retarded at a 0.4/1 PEI-LP/DNA weight ratio. In vitro delivery tests into isolated mitochondria or living cells were performed with rhodamin-labeled DNA and PEI-LP. In vitro cell-free delivery assay with isolated mitochondria showed that PEI-LP/DNA complexes were localized at mitochondria sites. Furthermore, the PEL-LP/DNA complexes were localized at the mitochondrial sites in living cells. However, a control carrier, PEI, did not show this effect. In addition, MTT assay showed that PEI-LP showed lower cytotoxicity than PEI. These results suggest that PEI-LP can deliver DNA to the mitochondrial sites and may be useful for the development of mitochondrial gene therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | DNA delivery to the mitochondria sites using mitochondrial leader peptide conjugated polyethylenimine | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1080/10611860600953555 | - |
dc.identifier.scopusid | 2-s2.0-34249714777 | - |
dc.identifier.wosid | 000244845000003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.15, no.2, pp.115 - 122 | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 15 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 115 | - |
dc.citation.endPage | 122 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | NONVIRAL GENE DELIVERY | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | BARRIERS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordAuthor | DNA delivery | - |
dc.subject.keywordAuthor | gene carrier | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.subject.keywordAuthor | mitochondrial leader peptide | - |
dc.subject.keywordAuthor | polyethylenimine | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/10611860600953555 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.