Phospholipase D1 as a key enzyme for decidualization in human endometrial stromal cells
DC Field | Value | Language |
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dc.contributor.author | Yoon, Mee-Sup | - |
dc.contributor.author | Koo, Jun Bon | - |
dc.contributor.author | Jeong, Yong Geon | - |
dc.contributor.author | Kim, Yong Seok | - |
dc.contributor.author | Lee, Jung Han | - |
dc.contributor.author | Yun, Hyae Jin | - |
dc.contributor.author | Lee, Ki Sung | - |
dc.contributor.author | Han, Joong-Soo | - |
dc.date.accessioned | 2022-12-21T09:13:48Z | - |
dc.date.available | 2022-12-21T09:13:48Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2007-02 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180504 | - |
dc.description.abstract | Using primary cell cultures of human endometrial stromal cells (ES cells), we investigated the role of phospholipase D (PLD) in 8-Br-cAMP-induced decidualization, which involves morphological and biological differentiation processes. When treated with 0.5 mM 8-Br-cAMP for 12 days, ES cells were transformed into a decidualized morphology and produced significant amounts of prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1). Simultaneously, the activity and expression levels of PLD1 increased. In addition, removal of 8-Br-cAMP from decidualized ES cells restored the undifferentiated state, and this was accompanied by decreases in PLD1 promoter activity and PLD1 expression. Overexpression of dominant negative (DN)-PLD1 inhibited the morphological changes induced by 0.5 mM 8-Br-cAMP, whereas PLD1 overexpression induced morphological changes in the absence of 0.5 mM 8-Br-cAMP treatment. Moreover, knockdown of PLD1 by siRNA and blockage of PLD by treatment with 0.3 % 1-butanol decreased PRL/IGFBP1 mRNA expression, whereas PLD1 overexpression increased PRL/IGFBP1 mRNA expression. Treatment of ES cells with phosphatidic acid (PA) for 3 days induced PRL mRNA expression and morphological changes, which implies that PA is an end-product of PLD activation-induced decidualization. In addition, pretreatment of ES cells with mepacrine decreased PRL/IGFBP1 expression and inhibited morphological change, whereas pretreatment with propranolol caused no changes, as compared to cAMP-treated cells, which suggests that PA induces decidualization through phospholipase A2 (PLA2G1B). Taken together, these results suggest that PLD1 regulates 8-Br-cAMP-induced decidualization through PLA2G1B, and that PLD1 upregulation is essential for the decidualization of ES cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.title | Phospholipase D1 as a key enzyme for decidualization in human endometrial stromal cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Yong Seok | - |
dc.contributor.affiliatedAuthor | Han, Joong-Soo | - |
dc.identifier.doi | 10.1095/biolreprod.106.056226 | - |
dc.identifier.scopusid | 2-s2.0-33846859540 | - |
dc.identifier.wosid | 000243615300010 | - |
dc.identifier.bibliographicCitation | BIOLOGY OF REPRODUCTION, v.76, no.2, pp.250 - 258 | - |
dc.relation.isPartOf | BIOLOGY OF REPRODUCTION | - |
dc.citation.title | BIOLOGY OF REPRODUCTION | - |
dc.citation.volume | 76 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 250 | - |
dc.citation.endPage | 258 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | MESSENGER-RNA EXPRESSION | - |
dc.subject.keywordPlus | STRESS FIBER FORMATION | - |
dc.subject.keywordPlus | D PLD ISOZYMES | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | PROLACTIN EXPRESSION | - |
dc.subject.keywordPlus | HL60 CELLS | - |
dc.subject.keywordPlus | CYCLIC-AMP | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordAuthor | 8-Br-cAMP | - |
dc.subject.keywordAuthor | developmental biology | - |
dc.subject.keywordAuthor | decidualization | - |
dc.subject.keywordAuthor | human endometrial stromal cell | - |
dc.subject.keywordAuthor | phospholipase | - |
dc.identifier.url | https://academic.oup.com/biolreprod/article/76/2/250/2629704 | - |
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