Cell sheet detachment from poly(N-vinylcaprolactam-co-N-isopropylacrylamide) grafted onto tissue culture polystyrene dishes
- Authors
- Lim, Youn Mook; Jeun, Joon Pyo; Lee, Joon Ho; Lee, Young Moo; Nho, Young Chang
- Issue Date
- Jan-2007
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- human fibroblast; poly(N-isopropylacrylamide); poly(N-vinylcaprolactam); hydration; cell detachment; cell culture
- Citation
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v.13, no.1, pp.21 - 26
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
- Volume
- 13
- Number
- 1
- Start Page
- 21
- End Page
- 26
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180583
- ISSN
- 1226-086X
- Abstract
- Fabrication of functional tissue constructs using sandwiched layers of cultured cells could prove to be an attractive approach to tissue engineering. Rapid detachment of cultured cell sheets is a very important recovery method that permits facile manipulation of the sheet and prevents functional damage. To induce hydrophilic and hydrophobic structural changes on the required culture substrate in response to a culture temperature alteration, poly(N-vinylcaprolactam-co-N-isopropylacrylamide) (PNVCL-co-PNIPAAm) was grafted onto tissue culture polystyrene (TCPS) dishes through electron beam irradiation. Chemical analyses using ATR-FTIR spectroscopy revealed that PNVCL-co-PNIPAAm was successfully grafted onto the surfaces of the TCPS dishes. The TCPS dishes grafted with PNVCL-co-PNIPAAm were utilized for the cell sheet detachment experiments. Approximately 150 min was required to detach most of the cell sheets from the PNVCL-co-PNIPAAm-grafted TCPS surfaces.
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