Plant sterol guggulsterone inhibits nuclear factor-kappa B signaling in intestinal epithelial cells by blocking I kappa B kinase and ameliorates acute murine colitis
DC Field | Value | Language |
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dc.contributor.author | Cheon, Jae Hee | - |
dc.contributor.author | Kim, Joo Sung | - |
dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Kim, Nayoung | - |
dc.contributor.author | Jung, Hyun Chae | - |
dc.contributor.author | Song, In Sung | - |
dc.date.accessioned | 2022-12-21T09:46:38Z | - |
dc.date.available | 2022-12-21T09:46:38Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2006-12 | - |
dc.identifier.issn | 1078-0998 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180696 | - |
dc.description.abstract | Background/Aims: The plant sterol guggulsterone has been shown to have anti-inflammatory properties. It remains unknown, however, whether guggulsterone is effective for the treatment of inflammatory bowel disease (IBD). Therefore, we investigated anti-inflammatory effects of guggulsterone on intestinal epithelial cells (IEC) and on experimental murine colitis models and elucidated its molecular mechanisms. Methods: Human Caco-2 cells and rat non-transformed IEC-18 cells were stimulated with interleukin (IL)-1 beta or lipopolysaccharide (LPS) with or without guggulsterone. The effects of guggulsterone on nuclear factor (NF)-kappa B signaling in IEC were examined by intercellular adhesion molecule (ICAM)-1 real-time reverse-transcription polymerase chain reaction, NF-kappa B transcriptional activity assay, Western blotting for I kappa B phosphorylation/degradation, electrophoretic mobility shift assay, and in vitro I kappa B kinase (IKK) assay For in vivo study, dextran sulfate sodium (DSS)-treated mice were fed with or without guggulsterone. Colitis was quantified by disease activity index and evaluation of macroscopic and microscopic findings. Phosphorylation Of I kappa B and IKK in colon mucosa was assessed by Western blotting and immunohistochemistry. Results: Guggulsterone significantly inhibited LPS- or IL-1 beta-induced ICAM-1 gene expression, NF-kappa B transcriptional activity, I kappa B phosphorylation/degradation, and NF-kappa B DNA binding activity in IEC. Moreover, guggulsterone strongly blocked IKK activity. Administration of guggulsterone significantly reduced the severity of DSS-induced murine colitis as assessed by clinical disease activity score, colon length, and histology Furthermore, tissue upregulation of I kappa B and IKK phosphorylation induced by DSS was attenuated in guggulsterone-treated mice. Conclusion: Guggulsterone blocks NF-kappa B signaling pathway by targeting IKK complex in IEC and attenuates DSS-induced acute murine colitis, which suggests that guggulsterone could be an attractive therapeutic option in the treatment of IBD. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.title | Plant sterol guggulsterone inhibits nuclear factor-kappa B signaling in intestinal epithelial cells by blocking I kappa B kinase and ameliorates acute murine colitis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.identifier.doi | 10.1097/01.mib.0000235830.94057.c6 | - |
dc.identifier.scopusid | 2-s2.0-33751577977 | - |
dc.identifier.wosid | 000242391600009 | - |
dc.identifier.bibliographicCitation | INFLAMMATORY BOWEL DISEASES, v.12, no.12, pp.1152 - 1161 | - |
dc.relation.isPartOf | INFLAMMATORY BOWEL DISEASES | - |
dc.citation.title | INFLAMMATORY BOWEL DISEASES | - |
dc.citation.volume | 12 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 1152 | - |
dc.citation.endPage | 1161 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | PROINFLAMMATORY GENE-EXPRESSION | - |
dc.subject.keywordPlus | TUMOR-NECROSIS-FACTOR | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSES | - |
dc.subject.keywordPlus | COMMIPHORA MUKUL | - |
dc.subject.keywordPlus | IKK-BETA | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordAuthor | guggulsterone | - |
dc.subject.keywordAuthor | nuclear factor-kappa B | - |
dc.subject.keywordAuthor | I kappa B kinase | - |
dc.subject.keywordAuthor | inflammatory bowel disease | - |
dc.identifier.url | https://academic.oup.com/ibdjournal/article/12/12/1152/4682864 | - |
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