Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography-tandem mass spectrometry
DC Field | Value | Language |
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dc.contributor.author | Cho, Sung-Hee | - |
dc.contributor.author | Jung, Byung Hwa | - |
dc.contributor.author | Lee, Sang Hee | - |
dc.contributor.author | Lee, Won-Yong | - |
dc.contributor.author | Kong, Gu | - |
dc.contributor.author | Chung, Bong Chul | - |
dc.date.accessioned | 2022-12-21T09:56:34Z | - |
dc.date.available | 2022-12-21T09:56:34Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2006-11 | - |
dc.identifier.issn | 0269-3879 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180786 | - |
dc.description.abstract | We developed an analytical method for a simple, sensitive and simultaneous determination of oxidized nucleosides in urine using column-switching liquid chromatography-electrospray/tandem mass spectrometry (LC-ESI/MS/MS). We connected two columns through a six-way switching valve and effectively separated nucleosides in the urine from the interference by column-switching liquid chromatography. We monitored separated nucleosides using positive ionization tandem mass spectrometry in selective reaction monitoring (SRM) mode. The calibration ranges of nucleosides were 0.2-100 nmol/mL. The linearity of the method was 0.994-0.999, and the limits-of-detection (LOD) at a signal-to-noise (S/N) ratio of 3 were 0.1-0.2 nmol/mL. The coefficients of variation were in the range 2.28-11.74% for within-day variation and 4.36-11.15% for day-to-day variation, respectively. To explore the relationship between breast cancer and the nucleosides level in human urine, we measured the concentrations of nucleosides in female patients with breast cancer (n = 30) and in normal female subjects (n = 30). The concentration of nucleosides was significantly increased in patients with breast cancer when compared with the normal controls (1-methyladenosine; p < 0.005, N-2,N-2-dimethylguanosine; p < 0.01, 5-hydroxymethyl-2'-deoxyuridine; p < 0.001, 8-hydroxy-2-deoxyguanosine; p < 0.001). Therefore, the elevated levels of nucleosides could be used as an important biomarker for breastcancer research. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.title | Direct determination of nucleosides in the urine of patients with breast cancer using column-switching liquid chromatography-tandem mass spectrometry | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kong, Gu | - |
dc.identifier.doi | 10.1002/bmc.689 | - |
dc.identifier.scopusid | 2-s2.0-33751075451 | - |
dc.identifier.wosid | 000242277400015 | - |
dc.identifier.bibliographicCitation | BIOMEDICAL CHROMATOGRAPHY, v.20, no.11, pp.1229 - 1236 | - |
dc.relation.isPartOf | BIOMEDICAL CHROMATOGRAPHY | - |
dc.citation.title | BIOMEDICAL CHROMATOGRAPHY | - |
dc.citation.volume | 20 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1229 | - |
dc.citation.endPage | 1236 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | OXIDATIVE DNA-DAMAGE | - |
dc.subject.keywordPlus | CEREBROSPINAL-FLUID | - |
dc.subject.keywordPlus | TRANSFER-RNA | - |
dc.subject.keywordPlus | PSEUDOURIDINE | - |
dc.subject.keywordPlus | LEUKEMIA | - |
dc.subject.keywordPlus | MARKERS | - |
dc.subject.keywordPlus | 5-HYDROXYMETHYL-2&apos | - |
dc.subject.keywordPlus | -DEOXYURIDINE | - |
dc.subject.keywordPlus | 8-HYDROXY-2-DEOXYGUANOSINE | - |
dc.subject.keywordPlus | 5-METHYL-2&apos | - |
dc.subject.keywordPlus | -DEOXYCYTIDINE | - |
dc.subject.keywordPlus | 8-HYDROXYGUANOSINE | - |
dc.subject.keywordAuthor | nucleosides | - |
dc.subject.keywordAuthor | column-switching liquid chromatography | - |
dc.subject.keywordAuthor | mass spectrometry | - |
dc.subject.keywordAuthor | urine | - |
dc.subject.keywordAuthor | breast cancer | - |
dc.identifier.url | https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bmc.689 | - |
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