Interleukin-10 plasmid construction and delivery for the prevention of type 1 diabetes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Minhyung | - |
dc.contributor.author | Park, Hyewon | - |
dc.contributor.author | Youn, Jeehee | - |
dc.contributor.author | Oh, Eun Taex | - |
dc.contributor.author | Ko, Kyungsoo | - |
dc.contributor.author | Kim, Sungwan | - |
dc.contributor.author | Park, Yongsoo | - |
dc.date.accessioned | 2022-12-21T10:11:30Z | - |
dc.date.available | 2022-12-21T10:11:30Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2006-10 | - |
dc.identifier.issn | 0077-8923 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/180925 | - |
dc.description.abstract | Studies of animals with spontaneous autoimmune diabetes have revealed that autoreactive T cells that mediate islet beta cell destruction can be manipulated by the administration of Th-2 cytokines. In this article, the effect of interleukin-10 (IL-10) gene delivery was evaluated in vitro and in vivo with a novel IL-10 plasmid, pSI-IL-10-NF kappa B. In PSI-IL-10-NF kappa B, the expression of the IL-10 gene was driven by the SV40 promotor/enhancer. The nuclear factor kappa B (NF kappa B) binding sites were also introduced to facilitate nuclear transport of the plasmid in the cell. In vitro transfection assay with pST-IL-10-NF kappa B showed a similar expression level of IL-10 to the plasmid without NF kappa B binding sites (pSI-IL-1 0). pSI-IL-10-NF kappa B and pSI-IL-10 were intravenously injected into 5-week-old nonobese diabetic (NOD) mice using polyethylenimine (PEI) as a gene carrier. Both groups had persistent gene expression, longer than 5 weeks, and secreted the similarly high IL-10 serum levels. Interestingly, the degree of insulitis in the pSI-IL-10-NF kappa B group was improved over the pSI-IL-10 group, PEI-only group, and noninjected controls. The serum glucose levels showed that single injection of pSI-IL-10-NF kappa B prevented the development of diabetes in 100% of the pSI-IL-10-NF kappa B-injected animals (5/5), while that of pSI-IL-10 prevented diabetes in 40% of the treated animals (2/5). These results suggest that pSI-IL-10-NF kappa B with PEI can effectively reduce the incidence of insulitis and type 1 diabetes in NOD mice. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BLACKWELL PUBLISHING | - |
dc.title | Interleukin-10 plasmid construction and delivery for the prevention of type 1 diabetes | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.contributor.affiliatedAuthor | Youn, Jeehee | - |
dc.contributor.affiliatedAuthor | Park, Yongsoo | - |
dc.identifier.doi | 10.1196/annals.1375.048 | - |
dc.identifier.scopusid | 2-s2.0-33845709923 | - |
dc.identifier.wosid | 000243126100048 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY OF DIABETES IV: PROGRESS IN OUR UNDERSTANDING, v.1079, pp.313 - 319 | - |
dc.relation.isPartOf | IMMUNOLOGY OF DIABETES IV: PROGRESS IN OUR UNDERSTANDING | - |
dc.citation.title | IMMUNOLOGY OF DIABETES IV: PROGRESS IN OUR UNDERSTANDING | - |
dc.citation.volume | 1079 | - |
dc.citation.startPage | 313 | - |
dc.citation.endPage | 319 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Proceedings Paper | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Endocrinology & Metabolism | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Endocrinology & Metabolism | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | AUTOIMMUNE INSULITIS | - |
dc.subject.keywordPlus | GENE-TRANSFER | - |
dc.subject.keywordPlus | KAPPA-B | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | IL-10 | - |
dc.subject.keywordAuthor | nonviral gene delivery | - |
dc.subject.keywordAuthor | NF kappa B binding sites | - |
dc.subject.keywordAuthor | type 1 diabetes | - |
dc.subject.keywordAuthor | NOD mouse | - |
dc.identifier.url | https://nyaspubs.onlinelibrary.wiley.com/doi/full/10.1196/annals.1375.048 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.