Bacteroides fragilis enterotoxin induces cyclooxygenase-2 and fluid secretion in intestinal epithelial cells through NF-kappa B activation
DC Field | Value | Language |
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dc.contributor.author | Kim, Jung Mogg | - |
dc.contributor.author | Lee, Jin Young | - |
dc.contributor.author | Yoon, Young Mee | - |
dc.contributor.author | Oh, Yu-Kyoung | - |
dc.contributor.author | Kang, Ju Seop | - |
dc.contributor.author | Kim, Yeong-Jeon | - |
dc.contributor.author | Kim, Kyoung-Ho | - |
dc.date.accessioned | 2022-12-21T10:34:42Z | - |
dc.date.available | 2022-12-21T10:34:42Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2006-09 | - |
dc.identifier.issn | 0014-2980 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181050 | - |
dc.description.abstract | Bacteroides fragilis produces an approximately 20-kDa heat-labile toxin (B. fragilis enterotoxin, BFT) which is known to be associated with diarrhea. To determine whether cyclooxygenase (COX)-2, via NF-kappa B activation, can contribute to BFT-induced diarrhea, the relationship between COX-2 expression and fluid secretion in BFT-stimulated human intestinal epithelial cells was examined. BFT stimulation increased the expression of COX-2, but not COX-1, in human intestinal epithelial cells. Suppression of the NF-kappa B signal significantly decreased COX-2 expression in response to BFT stimulation. Prostaglandin E-2 (PGE(2)) levels were increased in parallel with COX-2 expression, and, conversely, PGE(2) production was significantly inhibited when COX-2 or NF-kappa B activities were suppressed using COX-2 small interfering RNA (siRNA), p65 NF-kappa B subunit siRNA, or a retrovirus encoding the I kappa B alpha superrepressor. In addition, a selective COX-2 inhibitor, NS-398, significantly inhibited the increased cAMP level induced by BFT stimulation. Furthermore, a selective COX-2 inhibitor prevented BFT-induced PGE(2) production and ileal fluid secretion in a mouse ileal loop model. These results suggest that the secretory response to BFT stimulation may be mediated by the production of PGE(2), through NF-kappa B activation and the up-regulation of COX-2 in intestinal epithelial cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Bacteroides fragilis enterotoxin induces cyclooxygenase-2 and fluid secretion in intestinal epithelial cells through NF-kappa B activation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jung Mogg | - |
dc.contributor.affiliatedAuthor | Kang, Ju Seop | - |
dc.identifier.doi | 10.1002/eji.200535808 | - |
dc.identifier.scopusid | 2-s2.0-33748865503 | - |
dc.identifier.wosid | 000240586100019 | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF IMMUNOLOGY, v.36, no.9, pp.2446 - 2456 | - |
dc.relation.isPartOf | EUROPEAN JOURNAL OF IMMUNOLOGY | - |
dc.citation.title | EUROPEAN JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 36 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2446 | - |
dc.citation.endPage | 2456 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | HELICOBACTER-PYLORI | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | PROSTAGLANDIN E-2 | - |
dc.subject.keywordPlus | DIARRHEAL DISEASE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN-KINASE | - |
dc.subject.keywordPlus | UP-REGULATION | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordAuthor | Bacteroides fragilis enterotoxin | - |
dc.subject.keywordAuthor | cyclooxygenase-2 | - |
dc.subject.keywordAuthor | intestinal epithelial cells NF-kappa B | - |
dc.subject.keywordAuthor | prostaglandin E-2 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/eji.200535808 | - |
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