Low molecular weight polyethylenimine-mitochondrial leader peptide conjugate for DNA delivery to mitochondria
DC Field | Value | Language |
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dc.contributor.author | Choi, Joon Sig | - |
dc.contributor.author | Choi, Min Ji | - |
dc.contributor.author | Ko, Kyung Soo | - |
dc.contributor.author | Rhee, Byoung Doo | - |
dc.contributor.author | Pak, Youngmi Kim | - |
dc.contributor.author | Bang, In Seok | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-12-21T10:37:31Z | - |
dc.date.available | 2022-12-21T10:37:31Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2006-09 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181072 | - |
dc.description.abstract | It has been found that a number of diseases are associated with mutations in the mitochondrial DNA. Therapeutic gene delivery to mitochondria has been suggested as a clinical option for these diseases. In this study, we developed a gene carrier to mitochondria by the conjugation of mitochondrial leader peptide (LP) to polyethylenimine (PEI). Mitochondrial LP conjugated PEI (PEI-LP) was synthesized with low molecular weight PEI (2,000 Da, PEI2K). Gel retardation assay showed that PEI2K-LP formed complexes at a 1.0/1 weight ratio. In addition, PEI2K-LP protected DNA from the enzymatic degradation for at least 60 min, while naked DNA was completely degraded within 20 rum. PEI2K-LP was compared with LP conjugated high molecular weight PEI (25,000 Da, PEI25K) in terms of toxicity and delivery efficiency. MTT assay showed that PEI2K-LP had much lower cytotoxicity than PEI25K-LP to 293 cells. In addition, cell-free DNA delivery assay showed that PEI2K-LP delivered more DNA to mitochondria at a 1.8/1 weight ratio than naked DNA or PEI. This result suggests that PEI2K-LP may be useful for the development of mitochondrial gene therapy system with lower cytotoxicity. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN CHEMICAL SOC | - |
dc.title | Low molecular weight polyethylenimine-mitochondrial leader peptide conjugate for DNA delivery to mitochondria | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.5012/bkcs.2006.27.9.1335 | - |
dc.identifier.scopusid | 2-s2.0-33749023859 | - |
dc.identifier.wosid | 000241448300013 | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.27, no.9, pp.1335 - 1340 | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 27 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1335 | - |
dc.citation.endPage | 1340 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001192767 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DISEASES | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | PROTEOME | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | mitochondria | - |
dc.subject.keywordAuthor | leader peptide | - |
dc.subject.keywordAuthor | polyethylenimine | - |
dc.identifier.url | http://koreascience.or.kr/article/JAKO200602727181303.page | - |
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