N-acetyl histidine-conjugated glycol chitosan self-assembled nanoparticles for intracytoplasmic delivery of drugs: Endocytosis, exocytosis and drug release
DC Field | Value | Language |
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dc.contributor.author | Park, Ji Sun | - |
dc.contributor.author | Han, Tae Hee | - |
dc.contributor.author | Lee, Kuen Yong | - |
dc.contributor.author | Han, Sung Soo | - |
dc.contributor.author | Hwang, Jung Jin | - |
dc.contributor.author | Moon, Dae Hyuk | - |
dc.contributor.author | Kim, Sang Yoon | - |
dc.contributor.author | Cho, Yong Woo | - |
dc.date.accessioned | 2022-12-21T10:38:04Z | - |
dc.date.available | 2022-12-21T10:38:04Z | - |
dc.date.created | 2022-09-16 | - |
dc.date.issued | 2006-09 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181076 | - |
dc.description.abstract | Nano-sized vesicular systems (nanoparticles), ranging from 10 nm to 1000 nm in size, have potential applications as drug delivery systems. Successful clinical applications require the efficient intracellular delivery of drug-loaded nanoparticles. Here we describe N-acetyl histidine-conjugated glycol chitosan (NAcHis-GC) self-assembled nanoparticles as a promising system for intracytoplasmic delivery of drugs. Because N-acetyl histidine (NAcHis) is hydrophobic at neutral pH, the conjugates formed self-assembled nanoparticles with mean diameters of 150-250 nm. In slightly acidic environments, such as those in endosomes, the nanoparticles were disassembled due to breakdown of the hydrophilic/hydrophobic balance by the protonation of the imidazole group of NAcHis. Cellular internalization and drug release of the pH-sensitive self-assembled nanoparticles were investigated by flow cytometry and confocal microscopy. NAcHis-GC nanoparticles internalized by adsorptive endocytosis were exocytosed or localized in endosomes. The amount of exocytosed nanoparticles was dependent on the pre-incubation time prior to removal of free nanoparticles from the culture media. Flow cytometry and confocal microscopy showed that NAcHis-GC nanoparticles released drugs into the cytosol and cell cycle analysis demonstrated that paclitaxel-incorporated NAcHis-GC nanoparticles were effective in inducing arrest of cell growth. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.title | N-acetyl histidine-conjugated glycol chitosan self-assembled nanoparticles for intracytoplasmic delivery of drugs: Endocytosis, exocytosis and drug release | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kuen Yong | - |
dc.identifier.doi | 10.1016/j.jconrel.2006.07.011 | - |
dc.identifier.scopusid | 2-s2.0-33748785439 | - |
dc.identifier.wosid | 000241548400005 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CONTROLLED RELEASE, v.115, no.1, pp.37 - 45 | - |
dc.relation.isPartOf | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.title | JOURNAL OF CONTROLLED RELEASE | - |
dc.citation.volume | 115 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 37 | - |
dc.citation.endPage | 45 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | HISTIDYLATED POLYLYSINE | - |
dc.subject.keywordPlus | POLYMERIC MICELLES | - |
dc.subject.keywordPlus | DEPENDENT FUSION | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | PACLITAXEL | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | CARRIERS | - |
dc.subject.keywordPlus | PDNA | - |
dc.subject.keywordAuthor | nanoparticle | - |
dc.subject.keywordAuthor | histidine | - |
dc.subject.keywordAuthor | chitosan | - |
dc.subject.keywordAuthor | endocytosis | - |
dc.subject.keywordAuthor | exocytosis | - |
dc.subject.keywordAuthor | endosome | - |
dc.subject.keywordAuthor | paclitaxel | - |
dc.subject.keywordAuthor | cell cycle arrest | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0168365906003385?via%3Dihub | - |
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