Real-time PCR monitoring of Lactobacillus sake, Lactobacillus planiarum, and Lactobacillus paraplantarum during Kimchi fermentation

Abstract

Semi-quantitative monitoring of Lactobacillus sake and Lactobacillus plantarum, major and minor microorganisms in kimchi, respectively, and Lactobacillus paraplantarum, recently shown to be present in kimchi, was carried out by real-time polymerase chain reaction (PCR). Changes in the 3 species during kimchi fermentation were monitored by the threshold cycle (C-T) of real-time PCR. As fermentation proceeded at 15 degrees C, the number of L. sake increased dramatically compared to those of L. plantarum and L. paraplantarum. During fermentation at 4 degrees C, the growth rates of the 3 species decreased, but the proportions of L. plantarum and L. paraplantarum in the microbial ecosystem were almost constant. Considering the C-T values of the first samples and the change in the C-T value, the number of L. sake is no doubt greater than those of L. plantarum and L. paraplantarum in the kimchi ecosystem. L. sake seems to be one of the major microorganisms involved in kimchi fermentation, but there is insufficient evidence to suggest that L. plantarum is the primary acidifying bacterium.