Optimal temperature control in fractional precipitation for paclitaxel pre-purification
DC Field | Value | Language |
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dc.contributor.author | Jeon, Sun Im | - |
dc.contributor.author | Mun, Sung yong | - |
dc.contributor.author | Kim, Jin-Hyun | - |
dc.date.accessioned | 2022-12-21T12:02:46Z | - |
dc.date.available | 2022-12-21T12:02:46Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2006-02 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181781 | - |
dc.description.abstract | Fractional precipitation is a simple, efficient method for pre-purifying paclitaxel from plant cell cultures. The storage temperature of fractional precipitation was optimized in terms of the yield and purity of paclitaxel with a fixed methanol concentration in water (61.5%, v/v), paclitaxel content in the crude extract (0.5%, w/v), and storage time (3 days). The greatest yield (similar to 84%) was obtained with storage at a constant temperature (0 degrees C) for 3 days. Conversely, the highest purity (similar to 79%) was obtained with stepwise reduction in temperature over 3 days. For a constant storage temperature, the maximum purity (similar to 67%) was obtained at 0 degrees C. There was little difference in the yield of paclitaxel between -20 and 12 degrees C. At a constant storage temperature (0 degrees C), both the precipitate yield and purity increased rapidly up to 18 h, and then increased more slowly. This pre-purification process serves to minimize solvent usage, and the size and complexity of the high-performance liquid chromatography (HPLC) operation required for paclitaxel purification. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Optimal temperature control in fractional precipitation for paclitaxel pre-purification | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Mun, Sung yong | - |
dc.identifier.doi | 10.1016/j.procbio.2005.07.016 | - |
dc.identifier.scopusid | 2-s2.0-29244488578 | - |
dc.identifier.wosid | 000234624200005 | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.41, no.2, pp.276 - 280 | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 41 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 276 | - |
dc.citation.endPage | 280 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | CELL-CULTURES | - |
dc.subject.keywordPlus | TAXUS-BREVIFOLIA | - |
dc.subject.keywordPlus | TAXOL | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | CHINENSIS | - |
dc.subject.keywordPlus | NEEDLES | - |
dc.subject.keywordAuthor | paclitaxel | - |
dc.subject.keywordAuthor | fractional precipitation | - |
dc.subject.keywordAuthor | temperature | - |
dc.subject.keywordAuthor | pre-purification | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S1359511305003223?via%3Dihub | - |
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