Isolation of male germ stem cell-like cells from testicular tissue of non-obstructive azoospermic patients and differentiation into haploid male germ cells in vitro
DC Field | Value | Language |
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dc.contributor.author | Lee, Dong Ryul | - |
dc.contributor.author | Kim, Kye Seong | - |
dc.contributor.author | Yang, Yun Hee | - |
dc.contributor.author | Oh, Hwa Soon | - |
dc.contributor.author | Lee, Sook Hwan | - |
dc.contributor.author | Chung, Tae Gyu | - |
dc.contributor.author | Cho, Jung Hyun | - |
dc.contributor.author | Kim, Hyun Joo | - |
dc.contributor.author | Yoon, Tae Ki | - |
dc.contributor.author | Cha, Kwang Yul | - |
dc.date.accessioned | 2022-12-21T12:04:26Z | - |
dc.date.available | 2022-12-21T12:04:26Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2006-02 | - |
dc.identifier.issn | 0268-1161 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181798 | - |
dc.description.abstract | BACKGROUND: The purpose of this study was to establish the culture conditions required to isolate, identify and expand male germ stem cell-like cells (GSC-LC) from the testicular tissue of patients with non-obstructive azoospermia (NOA). METHODS AND RESULTS: Testicular tissues obtained from patients (two with maturation arrest (MA, n = 2) and Sertoli cell-only syndrome (SCOS, n = 11) were dissociated and plated into gelatin-coated dishes. After 2-4 weeks, cultures from both MA patients (100%) and four SCOS patients (36.3%) exhibited multicellular colonies, which proliferated successfully until passage 10. GSC-LC in the colonies displayed alkaline phosphatase activity, as well as Oct-4 and integrin b1 expression after every passage. After the fifth passage, GSC-LC were differentiated by encapsulation in calcium alginate and further cultivation. At 2 and 6 weeks, cells expressed c-Kit, Scp3, testis-specific histone protein 2B (TH2B), and transition protein (TP)-1. Fluorescence in situ hybridization additionally disclosed a few tetraploid and haploid cells at 6 weeks. Human oocytes were activated in the absence of artificial activation and cleaved after the injection of presumptive spermatids. CONCLUSIONS: Our novel culture system may be useful for diagnosing the existence of germ cells and facilitating the treatment of NOA patients. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Isolation of male germ stem cell-like cells from testicular tissue of non-obstructive azoospermic patients and differentiation into haploid male germ cells in vitro | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kye Seong | - |
dc.identifier.doi | 10.1093/humrep/dei319 | - |
dc.identifier.scopusid | 2-s2.0-31544478808 | - |
dc.identifier.wosid | 000234780400023 | - |
dc.identifier.bibliographicCitation | HUMAN REPRODUCTION, v.21, no.2, pp.471 - 476 | - |
dc.relation.isPartOf | HUMAN REPRODUCTION | - |
dc.citation.title | HUMAN REPRODUCTION | - |
dc.citation.volume | 21 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 471 | - |
dc.citation.endPage | 476 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Obstetrics & Gynecology | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Obstetrics & Gynecology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordPlus | SPERMATOGENESIS | - |
dc.subject.keywordPlus | MEIOSIS | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | SPERMIOGENESIS | - |
dc.subject.keywordPlus | COMPLETION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INJECTION | - |
dc.subject.keywordPlus | TESTIS | - |
dc.subject.keywordAuthor | embryo production | - |
dc.subject.keywordAuthor | germ stem cell-like cells | - |
dc.subject.keywordAuthor | haploid male germ cells | - |
dc.subject.keywordAuthor | non-obstructive azoospermic patients | - |
dc.subject.keywordAuthor | spermatogenesis in vitro | - |
dc.identifier.url | We would like to thank Jung Jin Lim, M.Sc. for providing us with the molecular work. This work was supported, in part, by a grant (SC2060) from the Stem Cell Research Center of the 21C Frontier R&D Program funded by the Ministry of Science and Technology, and a grant from the specialized program of the University funded by the Ministry of Education and Human Resources Development, Republic of Korea. | - |
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