Polydiacelylene (PDA)-based colorimetric detection of biotin-streptavidin interactions
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Yun Kyung | - |
dc.contributor.author | Park, Hyun Gyu | - |
dc.contributor.author | Kim, Jong Man | - |
dc.date.accessioned | 2022-12-21T12:05:29Z | - |
dc.date.available | 2022-12-21T12:05:29Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2006-02 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/181808 | - |
dc.description.abstract | The natural binding of streptavidin (STA) for a small molecule, biotin, has made it a useful tool in specific targeting application, due to their most specific noncovalent biological interactions in nature. Here, we describe a polydiacetylene (PDA)-based colorimetric biosensor to detect the STA-biotin interactions. We synthesized two kinds of biotin-labeled 10,12-pentacosadiynoic acid (PCDA) monomers having 2(ethylene oxide) or 3(ethylene oxide) spacer and prepared biotin-modified PDA liposomes using them. Upon the addition of STA, the modified PDA liposomes showed a color change from blue to red in about I h followed by their aggregation and precipitation. The aggregation was found to be due to the cross-linking between the PDA liposomes caused by the STA having four biotin binding sites, which was confirmed by their TEM analysis. Since the STA-biotin interactions are widely adopted for various biological detection systems; the biotin-modified PDA sensor developed in this study has enormous potentials for the development of various colorimetric biosensors. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Polydiacelylene (PDA)-based colorimetric detection of biotin-streptavidin interactions | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jong Man | - |
dc.identifier.doi | 10.1016/j.bios.2005.07.010 | - |
dc.identifier.scopusid | 2-s2.0-31044450823 | - |
dc.identifier.wosid | 000235492900017 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.21, no.8, pp.1536 - 1544 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 21 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1536 | - |
dc.citation.endPage | 1544 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | MOLECULAR RECOGNITION | - |
dc.subject.keywordPlus | INTERFACIAL CATALYSIS | - |
dc.subject.keywordPlus | LIPID VESICLES | - |
dc.subject.keywordPlus | POLYMERIZATION | - |
dc.subject.keywordPlus | NANOCOMPOSITES | - |
dc.subject.keywordPlus | LIPOSOMES | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordAuthor | polydiacetylene | - |
dc.subject.keywordAuthor | biotin | - |
dc.subject.keywordAuthor | streptavidin | - |
dc.subject.keywordAuthor | colorimetric biosensors | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S095656630500206X?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.