Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches
DC Field | Value | Language |
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dc.contributor.author | Kwon, Jeonghun | - |
dc.contributor.author | Kim, Minyoung | - |
dc.contributor.author | Hwang, Woochang | - |
dc.contributor.author | Jo, Anna | - |
dc.contributor.author | Hwang, Gue-Ho | - |
dc.contributor.author | Jung, Minhee | - |
dc.contributor.author | Kim, Un Gi | - |
dc.contributor.author | Cui, Gang | - |
dc.contributor.author | Kim, Heonseok | - |
dc.contributor.author | Eom, Joon-Ho | - |
dc.contributor.author | Hur, Junho K. | - |
dc.contributor.author | Lee, Junwon | - |
dc.contributor.author | Kim, Youngho | - |
dc.contributor.author | Kim, Jin-soo | - |
dc.contributor.author | Bae, Sangsu | - |
dc.contributor.author | Lee, Jungjoon K. | - |
dc.date.accessioned | 2023-02-21T05:33:02Z | - |
dc.date.available | 2023-02-21T05:33:02Z | - |
dc.date.created | 2023-02-08 | - |
dc.date.issued | 2023-01 | - |
dc.identifier.issn | 1474-760X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/182356 | - |
dc.description.abstract | We present a novel genome-wide off-target prediction method named Extru-seq and compare it with cell-based (GUIDE-seq), in vitro (Digenome-seq), and in silico methods using promiscuous guide RNAs with large numbers of valid off-target sites. Extru-seq demonstrates a high validation rate and retention of information about the intracellular environment, both beneficial characteristics of cell-based methods. Extru-seq also shows a low miss rate and could easily be performed in clinically relevant cell types with little optimization, which are major positive features of the in vitro methods. In summary, Extru-seq shows beneficial features of cell-based and in vitro methods. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.title | Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hwang, Woochang | - |
dc.contributor.affiliatedAuthor | Hur, Junho K. | - |
dc.identifier.doi | 10.1186/s13059-022-02842-4 | - |
dc.identifier.scopusid | 2-s2.0-85146106669 | - |
dc.identifier.wosid | 000911951200001 | - |
dc.identifier.bibliographicCitation | GENOME BIOLOGY, v.24, no.1, pp.1 - 20 | - |
dc.relation.isPartOf | GENOME BIOLOGY | - |
dc.citation.title | GENOME BIOLOGY | - |
dc.citation.volume | 24 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 20 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | UNBIASED DETECTION | - |
dc.subject.keywordPlus | WEB TOOL | - |
dc.subject.keywordPlus | CRISPR-CAS9 | - |
dc.subject.keywordPlus | REARRANGEMENTS | - |
dc.subject.keywordPlus | INTEGRATION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordAuthor | CRISPR | - |
dc.subject.keywordAuthor | Genome-wide | - |
dc.subject.keywordAuthor | Off-target | - |
dc.subject.keywordAuthor | Cell-based | - |
dc.subject.keywordAuthor | In vitro | - |
dc.identifier.url | https://genomebiology.biomedcentral.com/articles/10.1186/s13059-022-02842-4 | - |
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